LOCUS       sequence-283815-        2081 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0994
FEATURES             Location/Qualifiers
     source          1..2081
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagcggc ggtgctgatg gaccgggttc agagttgtgt gaccttcgag
       61 gatgtgttcg tgtacttctc tcgggaggag tgggaactcc ttgaggaggc acagagattc
      121 ctgtaccgtg atgtgatgct ggagaacttt gcacttgtgg ctacactagg tttttggtgt
      181 gaagcagaac atgaggcacc ttctgagcag agcgtttctg tagaaggagt gtcacaggtc
      241 aggactgctg agtcaggtct tttccagaaa gcacacccat gtgagatgtg tgacccactc
      301 ttgaaagaca ttttgcacct ggctgaacac cagggatcac accttacaca gaaactgtgc
      361 acacgtgggc tgtgtaggag aagattctcg ttcagtgcaa acttttacca gcaccagaag
      421 caacataatg gagagaattg cttcagaggg gatgatggag gggcctcatt tgtgaagagc
      481 tgtacagtcc acatgttagg gagatccttt acgtgcaggg aggaagggat ggacttacca
      541 gatagctctg gccttttcca gcaccagacc acttacaata gggtgagtcc atgcagaagg
      601 actgaatgca tggagtcttt cccacacagc tccagtctca ggcaacacca aggagactat
      661 gatggacaga tgcttttcag ttgcggtgat gaagggaaag ccttcctgga cacctttact
      721 cttcttgaca gccagatgac tcatgctgag gtgagaccct tcagatgcct accatgtgga
      781 aatgtgttca aggagaaatc agctcttatt aatcacagaa aaatccacag tggagaaata
      841 tctcatgtgt gtaaggagtg tggaaaagcc ttcattcact tgcaccacct aaaaatgcac
      901 cagaaatttc acactggaaa aagacactat acatgcagtg aatgtgggaa ggccttcagc
      961 cgcaaggaca cacttgttca gcatcagaga gttcacactg gagaaagatc ttatgactgc
     1021 agtgaatgtg gaaaagccta cagcagaagc tcccaccttg ttcagcacca gagaattcac
     1081 acaggagaaa ggccttataa gtgcaacaaa tgtgggaaag cctttagccg taaagacaca
     1141 cttgttcagc accagagatt tcatactgga gaaaggcctt atgagtgcag tgaatgtgga
     1201 aaattcttta gccaaagctc ccaccttatt gagcactgga gaattcatac cggggcaagg
     1261 ccctatgaat gcatagaatg tggaaaattc tttagccata actctagcct cattaaacat
     1321 cggagagtcc acacaggagc aagatcctac gtgtgcagca aatgtgggaa ggcctttggc
     1381 tgcaaagaca cacttgttca gcaccagata attcacactg gagcaaggcc ttatgagtgc
     1441 agtgaatgtg ggaaggcctt cagccgtaaa gacacacttg tgcaacacca aaaaatccac
     1501 actggagaaa ggccttatga gtgtggtgaa tgtggtaaat tcttcagcca tagctccaac
     1561 cttattgtac accagagaat tcacactgga gcaaagcctt atgagtgcaa tgaatgtggg
     1621 aaatgcttta gccacaactc cagcctcatt ttgcaccaga gagttcacac aggagcaagg
     1681 ccttatgtgt gcagtgaatg tgggaaggct tacattagta gctcccacct tgttcaacac
     1741 aagaaagttc acactggagc aagaccttat gagtgcagtg aatgtgggaa attctttagc
     1801 cgcaactctg gcctcattct gcaccagagg gttcacactg gagaaaagcc ttacgtatgc
     1861 agcgaatgtg ggaaagccta tagcagaagc tcccatcttg ttcgtcacca gaaagctcac
     1921 actggagaaa gagctcacga gtgcaacagt tttggtggcc ctttagctgc atctcttaaa
     1981 cttgtttagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     2041 tcttgtggaa aggacgagca tttgaaactc ctcagatggt g
//
