LOCUS       sequence-283830-        2084 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1015
FEATURES             Location/Qualifiers
     source          1..2084
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcccatgc attggtgacg ttcagggatg tggctataga cttctctcag
       61 aaggaatggg agtgcctgga cactacccag aggaaattgt acagagatgt gatgttggag
      121 aattataata acttggtctc actgggatat tctggctcaa agccagatgt gattacctta
      181 ctggagcaag ggaaagagcc ctgcgtggtg gcgagggatg tgacaggaag acagtgcccc
      241 ggtttgttat ccaggcataa gaccaagaaa ttatcttcag aaaaggacat tcatgaaatc
      301 agtttatcca aagagagtat aatagaaaaa agtaaaactc ttcgtctgaa aggatccatt
      361 tttagaaatg agtggcagaa caaaagtgag tttgagggtc aacagggact taaagaaaga
      421 tctatcagtc aaaagaaaat cgtctctaaa aaaatgtcaa ctgatagaaa acgtccctct
      481 tttactctga atcagagaat tcacaatagt gagaaaagct gtgactcaca cttggttcaa
      541 catgggaaaa tagattctga tgtgaaacat gattgtaaag aatgtgggag tacttttaat
      601 aatgtctatc agcttactct ccatcagaaa attcatactg gtgaaaaatc ctgtaaatgt
      661 gagaaatgtg ggaaagtttt tagtcatagc tatcaactta ctctgcatca gagatttcat
      721 actggtgaga aaccctatga atgtcaagaa tgtgggaaga cctttactct ttacccacaa
      781 cttaatcgac atcagaaaat tcacactggt aaaaaaccct atatgtgtaa gaaatgtgat
      841 aagggttttt ttagtagatt agaacttact caacataaaa gaattcatac tggtaagaaa
      901 tcttatgaat gtaaagaatg tggaaaagtt tttcaactta ttttctactt taaagaacat
      961 gagagaattc atacaggtaa gaaaccctat gaatgtaagg agtgtgggaa agcttttagt
     1021 gtatgcggac aacttacccg tcatcagaaa attcatactg gtgtaaaacc ctacgaatgt
     1081 aaggaatgtg gaaagacctt tagacttagt ttttacctta ctgaacacag aagaactcat
     1141 gcaggtaaga aaccttatga atgtaaggag tgtgggaaat catttaatgt gcgtggacag
     1201 cttaatcggc ataaaacaat ccatactggt ataaaacctt ttgcatgtaa ggtgtgtgag
     1261 aaggctttta gttatagtgg tgacctcaga gtacattcta gaattcatac tggagagaaa
     1321 ccatatgaat gtaaggaatg cgggaaagcc tttatgcttc gttcagtcct tactgaacat
     1381 cagagacttc atactggtgt gaagccctac gaatgtaagg aatgtgggaa gacctttcga
     1441 gttcgttctc aaattagtct acataagaaa attcatactg atgtgaagcc ctacaaatgt
     1501 gtacgatgtg ggaagacctt tagatttggt ttctacctta ctgaacacca gagaattcac
     1561 actggtgaaa agccctataa atgtaaagaa tgtggaaagg cctttattcg tagagggaat
     1621 cttaaagaac atctgaaaat tcattctggt ttaaaaccct atgactgtaa agaatgtggg
     1681 aagtccttta gtcggcgtgg gcagttcact gaacatcaga aaattcatac gggtgtaaaa
     1741 ccatacaaat gtaaagaatg tgggaaggcc tttagtcgta gtgtagacct tagaatacat
     1801 caaagaattc atactggtga gaaaccctat gagtgtaaac aatgtgggaa ggcctttaga
     1861 cttaattcac accttactga acatcagaga attcacactg gtgagaaacc ctatgagtgt
     1921 aaggtatgta gaaaggcctt tagacaatat tcacatcttt atcaacatca gaaaactcat
     1981 aatgtaattt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     2041 atatcttgtg gaaaggacga cgaaggagta ttgagacagc gggc
//
