LOCUS       sequence-283833-        1985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1016
FEATURES             Location/Qualifiers
     source          1..1985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccgaggc agcgctagtg attacgccgc agggccatgt gacctttgag
       61 gatattgctg tgtacttctc ccaggaggag tggggcctcc ttgatgaagc tcaaaggtgc
      121 ctgtatcatg atgtgatgct ggagaacttt tcgcttatgg cctcagtagg ttgtttgcat
      181 ggaatagagg ctgaggaggc cccttctgag cagactcttt ctgcgcaagg agtgtcacag
      241 gccaggactc caaagctagg tccttccatc ccaaatgctc attcttgtga gatgtgtatc
      301 ctggtcatga aagacatttt gtacctcagt gagcatcagg ggacacttcc ctggcagaaa
      361 ccttatacgt ctgtggccag tgggaaatgg ttttcatttg gttctaacct gcaacagcac
      421 cagaaccagg acagtggaga gaaacacatc agaaaggagg agagcagtgc cttgcttctg
      481 aatagctgca aaattcctct gtcagacaat cttttcccat gcaaagatgt tgagaaggat
      541 tttccaacca tcctgggcct tctccaacac cagaccaccc acagcagaca agagtatgca
      601 catagaagca gggagacctt tcaacaaaga cgttacaaat gtgagcaagt tttcaatgag
      661 aaagttcatg ttactgagca tcagagagtc cacactggag aaaaagctta taagcgtagg
      721 gaatatggga aatccttgaa ctctaaatac ttatttgttg aacaccagag aacccataat
      781 gcagaaaagc cttatgtgtg caatatatgt gggaaatcat tcctccataa acaaacactc
      841 gttgggcacc agcagagaat tcacactaga gaaaggtctt atgtgtgcat cgaatgtggg
      901 aaatccttga gctccaaata ctcacttgtg gaacaccaga gaacccataa tggagaaaag
      961 ccttatgtgt gcaatgtatg tgggaaatca ttccgccaca aacaaacatt tgttggccat
     1021 cagcagagaa tccacactgg agagaggcct tatgtgtgta tggaatgtgg gaaatctttt
     1081 attcattcct atgaccgcat tcgacaccag agagttcaca ctggagaagg ggcttatcag
     1141 tgcagtgaat gtgggaaatc cttcatatac aaacagtcac ttcttgatca ccatagaatc
     1201 cacacgggag aaaggcctta tgagtgcaaa gaatgtggga aggccttcat tcacaaaaaa
     1261 agacttcttg agcaccagag aattcatact ggagaaaagc cttatgtgtg catcatatgt
     1321 gggaaatcat ttatccgctc gtctgactac atgcgacacc agagaattca cactggagaa
     1381 agggcttatg aatgcagtga ctgtgggaaa gccttcatct ccaaacaaac acttcttaag
     1441 catcacaaaa tccacactag agaaaggcct tatgaatgca gtgaatgtgg aaaaggcttc
     1501 taccttgagg ttaaacttct tcagcaccaa agaatccata ctagagaaca actttgtgag
     1561 tgcaatgaat gtggaaaagt cttcagccac caaaaaagac ttcttgagca ccagaaagtt
     1621 cacactggcg aaaagccctg tgagtgcagt gaatgtggga aatgctttag acaccgcacc
     1681 agcctcattc aacaccagaa agttcacagt ggagagaggc cttataactg cactgcatgt
     1741 gagaaggcct ttatctataa aaacaaactt gttgagcatc agcgaatcca caccggagaa
     1801 aagccgtatg aatgtggtaa atgtgggaaa gccttcaaca aaagatattc ccttgtcagg
     1861 caccagaagg tacatataac agaagagccc tagtaatgaa ctagtccgta acttgaaagt
     1921 atttcgattt cttggcttta tatatcttgt ggaaaggacg acgctattta cgactagacc
     1981 gtgga
//
