LOCUS       sequence-283842-        2678 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1020
FEATURES             Location/Qualifiers
     source          1..2678
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacctccc ggcgctgctc cccgccccga ctgcgcgcgg agggcaacat
       61 ggcggcggcc ccggcccgct ccgccgagcc ccagcgccgc tcggcgcgag ccccgcgcgc
      121 cgccgcctgc tactggtgcg gggccctgaa gatggcgggc ccggggcgcg gcccggggag
      181 gcctccgggc caagcccgcc gcccgccgag gacgacagcg acggcgactc tttcttggtg
      241 ctgctggaag tgccgcacgg cggcgctgcc gccgaggctg ccggatcaca ggaggccgag
      301 cctggctccc gtgtcaacct ggcgagccgc cccgagcagg gccccagcgg cccggccgcc
      361 ccccccggcc ctggcgtagc cccggcgggc gccgtcacca tcagcagcca ggacctgctg
      421 gtgcgtctcg accgcggcgt cctcgcgctg tctgcgccgc ccggccccgc aaccgcgggc
      481 gccgccgctc cccgccgcgc gccccaggcc tccggcccca gcacgcccgg ctaccgctgc
      541 cccgagccgc agtgcgcgct ggccttcgcc aagaagcacc agctcaaggt gcacctgctc
      601 acgcacggcg gcggtcaggg ccggcggccc ttcaagtgcc cactggaggg ctgtggttgg
      661 gccttcacaa cgtcctacaa gctcaagcgg cacctgcagt cgcacgacaa gctgcggccc
      721 ttcggctgtc cagtgggcgg ctgtggcaag aagttcacta cggtctataa cctcaaggcg
      781 cacatgaagg gccacgagca ggagagcctg ttcaagtgcg aggtgtgcgc cgagcgcttc
      841 cccacgcacg ccaagctcag ctcccaccag cgcagccact tcgagcccga gcgcccttac
      901 aagtgtgact ttcccggctg tgagaagaca tttatcacag tgagtgccct gttttcccat
      961 aaccgagccc acttcaggga acaagagctc ttttcctgct cctttcctgg gtgcagcaag
     1021 cagtatgata aagcctgtcg gctgaaaatt cacctgcgga gccatacagg tgaaagacca
     1081 tttatttgtg actctgacag ctgtggctgg accttcacca gcatgtccaa acttctaagg
     1141 cacagaagga aacatgacga tgaccggagg tttacctgcc ctgtcgaggg ctgtgggaaa
     1201 tcattcacca gagcagagca tctgaaaggc cacagcataa cccacctagg cacaaagccg
     1261 ttcgagtgtc ctgtggaagg atgttgcgcg aggttctccg ctcgtagcag tctgtacatt
     1321 cactctaaga aacacgtgca ggatgtgggt gctccgaaaa gccgttgccc agtttctacc
     1381 tgcaacagac tcttcacctc caagcacagc atgaaggcgc acatggtcag acagcacagc
     1441 cggcgccaag atctcttacc tcagctagaa gctccgagtt ctcttactcc cagcagtgaa
     1501 ctcagcagcc caggccaaag tgagctcact aacatggatc ttgctgcact cttctctgac
     1561 acacctgcca atgctagtgg ttctgcaggt gggtcggatg aggctctgaa ctccggaatc
     1621 ctgactattg acgtcacttc tgtgagctcc tctctgggag ggaacctccc tgctaataat
     1681 agctccctag ggccgatgga acccctggtc ctggtggccc acagtgatat tcccccaagc
     1741 ctggacagcc ctctggttct cgggacagca gccacggttc tgcagcaggg cagcttcagt
     1801 gtggatgacg tgcagactgt gagtgcagga gcattaggct gtctggtggc tctgcccatg
     1861 aagaacttga gtgacgaccc actggctttg acctccaata gtaacttagc agcacatatc
     1921 accacaccga cctcttcgag caccccccga gaaaatgcca gtgtcccgga actgctggct
     1981 ccaatcaagg tggagccgga ctcgccttct cgcccaggag cagttgggca gcaggaagga
     2041 agccatgggc tgccccagtc cacgttgccc agtccagcag agcagcacgg tgcccaggac
     2101 acagagctca gtgcaggcac tggcaacttc tatttggaaa gtgggggctc agcaagaact
     2161 gattaccgag ccattcaact agccaaggaa aaaaagcaga gaggagcggg gagcaatgca
     2221 ggagcctcac agtctactca gagaaaaata aaagaaggca aaatgagtcc tccccatttc
     2281 catgcaagcc agaacagttg gttgtgtggg agcctcgtgg tgcccagcgg aggacggcca
     2341 ggaccagctc cagcagctgg ggtgcagtgc ggggcgcagg gcgtccaggt ccagctggtg
     2401 caggatgacc cctccggcga aggtgtcctg ccctcggccc gcggcccagc caccttcctc
     2461 cccttcctca ctgtggacct gcccgtctac gtcctccagg aggtgctccc ctcatctgga
     2521 ggccctgctg gaccggaggc cacccagttc ccaggaagca ctatcaacct gcaggatctg
     2581 cagtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     2641 tgtggaaagg acgaatccca taactggcgt ttgtagag
//
