LOCUS       sequence-283854-        2081 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1051
FEATURES             Location/Qualifiers
     source          1..2081
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             785..793
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 accgccacca tgagggtcaa ccacacagtg tctacaatgc tccctacatg catggttcac
       61 agacaaacca tgagttgttc cggggctgga ggaataacgg cgttcgtggc attcagggat
      121 gtggctgtgt acttcaccca ggaggagtgg aggttgttga gccctgctca gaggaccctg
      181 cacagggagg tgatgctgga gacttataac catctggtct cactggaaat tccatcttct
      241 aaaccaaaac tcattgctca gctggagcga ggggaagcgc cctggagaga ggagagaaaa
      301 tgtccactgg acctctgtcc agaatcgaag ccagaaattc aacttagtcc ctcctgccct
      361 ctgattttct ccagtcagca agctctcagc caacatgtgt ggctgagtca tctctctcag
      421 ctgttttcaa gtttatgggc aggaaatcct ctccacctgg gaaaacacta tccagaagat
      481 cagaaacaac agcaggatcc attctgcttt agtggcaaag cagaatggat tcaagaggga
      541 gaagactcca gactcctgtt tgggagagta agcaaaaatg gcacttcaaa ggcactttcc
      601 agcccacctg aagaacaaca gccagcacag tccaaggaag acaacacagt ggtggatata
      661 gggtccagcc ctgaacggag ggcagatcta gaggaaacag acaaagtatt gcatggttta
      721 gaagtctcag gatttggaga aatcaaatat gaagagtttg ggccaggctt tatcaaggag
      781 tcaaacctcc ttagcctcca gaagacacaa actggggaga caccttacat gtacactgag
      841 tggggagaca gctttggcag tatgtcagtc ctcatcaaaa acccaaggac acactctggg
      901 ggaaagcctt atgtgtgcag ggaatgtggg cgaggcttta cgtggaagtc aaacctgatc
      961 acacatcaga ggacacactc aggggagaaa ccttatgtgt gcaaggattg tggacgaggc
     1021 tttacttgga agtcgaacct ctttacacat cagcggacac actcagggct caagccttat
     1081 gtgtgcaagg aatgtgggca gagctttagc ctgaagtcaa acctcattac ccaccagagg
     1141 gcgcacactg gggagaagcc ttatgtttgc agggaatgtg ggcgtggctt tcgccagcat
     1201 tcacacctgg tcagacacaa gaggacacat tcaggagaga agccttacat ttgcagggag
     1261 tgtgagcaag gctttagcca gaagtcacac ctcatcagac acttaaggac acacacagga
     1321 gagaagcctt atgtatgcac agaatgtggg cgtcacttta gctggaaatc aaacctcaaa
     1381 acacaccaga ggacacactc aggggttaaa ccttatgtct gcctggagtg cgggcagtgc
     1441 tttagcctga agtcaaacct taacaaacac cagaggtcac acacggggga gaagccattt
     1501 gtatgtacgg agtgtgggcg aggctttacc cggaaatcaa ccctgagcac gcaccagagg
     1561 acacactcag gggagaagcc atttgtatgt gctgagtgtg gacgaggctt taatgataag
     1621 tccaccctca tttcacacca gaggacacat tcaggggaaa agccttttat gtgcagggag
     1681 tgtggcagaa ggtttcggca gaagcctaac ctgtttaggc acaagagggc acactcaggt
     1741 gcctttgtgt gcagggagtg tgggcaaggc ttttgtgcta agttaactct cattaaacac
     1801 cagagagcac acgcaggggg gaagcctcat gtgtgcaggg agtgtgggca aggctttagc
     1861 cggcagtcac acctcattag acaccagagg acacattcag gagagaagcc ttatatttgc
     1921 agaaagtgtg gacggggctt tagtcggaag tccaacctta tcagacatca gaggacacac
     1981 tcaggatagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     2041 tcttgtggaa aggacgatga agacctcgcg cgctgcagtg t
//
