LOCUS       sequence-283857-        2024 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1052
FEATURES             Location/Qualifiers
     source          1..2024
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             728..736
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 accgccacca tggccacagg gctcctgaga gccaaaaaag aggcgttcgt ggcattcagg
       61 gatgtggctg tgtacttcac ccaggaggag tggaggttgt tgagccctgc tcagaggacc
      121 ctgcacaggg aggtgatgct ggagacttat aaccatctgg tctcactgga aattccatct
      181 tctaaaccaa aactcattgc tcagctggag cgaggggaag cgccctggag agaggagaga
      241 aaatgtccac tggacctctg tccagaatcg aagccagaaa ttcaacttag tccctcctgc
      301 cctctgattt tctccagtca gcaagctctc agccaacatg tgtggctgag tcatctctct
      361 cagctgtttt caagtttatg ggcaggaaat cctctccacc tgggaaaaca ctatccagaa
      421 gatcagaaac aacagcagga tccattctgc tttagtggca aagcagaatg gattcaagag
      481 ggagaagact ccagactcct gtttgggaga gtaagcaaaa atggcacttc aaaggcactt
      541 tccagcccac ctgaagaaca acagccagca cagtccaagg aagacaacac agtggtggat
      601 atagggtcca gccctgaacg gagggcagat ctagaggaaa cagacaaagt attgcatggt
      661 ttagaagtct caggatttgg agaaatcaaa tatgaagagt ttgggccagg ctttatcaag
      721 gagtcaaacc tccttagcct ccagaagaca caaactgggg agacacctta catgtacact
      781 gagtggggag acagctttgg cagtatgtca gtcctcatca aaaacccaag gacacactct
      841 gggggaaagc cttatgtgtg cagggaatgt gggcgaggct ttacgtggaa gtcaaacctg
      901 atcacacatc agaggacaca ctcaggggag aaaccttatg tgtgcaagga ttgtggacga
      961 ggctttactt ggaagtcgaa cctctttaca catcagcgga cacactcagg gctcaagcct
     1021 tatgtgtgca aggaatgtgg gcagagcttt agcctgaagt caaacctcat tacccaccag
     1081 agggcgcaca ctggggagaa gccttatgtt tgcagggaat gtgggcgtgg ctttcgccag
     1141 cattcacacc tggtcagaca caagaggaca cattcaggag agaagcctta catttgcagg
     1201 gagtgtgagc aaggctttag ccagaagtca cacctcatca gacacttaag gacacacaca
     1261 ggagagaagc cttatgtatg cacagaatgt gggcgtcact ttagctggaa atcaaacctc
     1321 aaaacacacc agaggacaca ctcaggggtt aaaccttatg tctgcctgga gtgcgggcag
     1381 tgctttagcc tgaagtcaaa ccttaacaaa caccagaggt cacacacggg ggagaagcca
     1441 tttgtatgta cggagtgtgg gcgaggcttt acccggaaat caaccctgag cacgcaccag
     1501 aggacacact caggggagaa gccatttgta tgtgctgagt gtggacgagg ctttaatgat
     1561 aagtccaccc tcatttcaca ccagaggaca cattcagggg aaaagccttt tatgtgcagg
     1621 gagtgtggca gaaggtttcg gcagaagcct aacctgttta ggcacaagag ggcacactca
     1681 ggtgcctttg tgtgcaggga gtgtgggcaa ggcttttgtg ctaagttaac tctcattaaa
     1741 caccagagag cacacgcagg ggggaagcct catgtgtgca gggagtgtgg gcaaggcttt
     1801 agccggcagt cacacctcat tagacaccag aggacacatt caggagagaa gccttatatt
     1861 tgcagaaagt gtggacgggg ctttagtcgg aagtccaacc ttatcagaca tcagaggaca
     1921 cactcaggat agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1981 atatcttgtg gaaaggacga acacacctat gcggtgggac ttac
//
