LOCUS       sequence-28386-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP46
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             31..40
                     /estimated_length=10
     primer_bind     45..67
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     83..99
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      128..137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             137..160
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             173..199
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    215..239
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ccctaagcct ccgcctcctc ttcctccatc nnnnnnnnnn ctcccccttg aacctcctcg
       61 ttcgaccccg cctcgatcct ccctttatcc agccctcact ccttctctag gcgccggaat
      121 tagatctgcc accatggact acaaggatga cgatgacaag ctcgatggag gatacccata
      181 cgatgttcca gattacgctg ctcgaggtta tcaaacaagt ttgtacaaaa aagttggcat
      241 gactgtccga aacatcgcct ccatctgtaa tatgggcacc aatgcctctg ctctggaaaa
      301 agacattggt ccagagcagt ttccaatcaa tgaacactat ttcggattgg tcaattttgg
      361 aaacacatgc tactgtaact ccgtgcttca ggcattgtac ttctgccgtc cattccggga
      421 gaatgtgttg gcatacaagg cccagcaaaa gaagaaggaa aacttgctga cgtgcctggc
      481 ggaccttttc cacagcattg ccacacagaa gaagaaggtt ggcgtcatcc caccaaagaa
      541 gttcatttca aggctgagaa aagagaatga tctctttgat aactacatgc agcaggatgc
      601 tcatgaattt ttaaattatt tgctaaacac tattgcggac atccttcagg aggagaagaa
      661 acaggaaaaa caaaatggaa aattaaaaaa tggcaacatg ancgaacctg cggaaaataa
      721 taaaccagaa ctcacctggg tccatgagan ttttcaggga acgcttacca ntgaaactcg
      781 atgcntngaa ctgtgaaact gtnantagca aanatgaaga ttttcntgac ctttct
//
