LOCUS       sequence-28387-e         654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP48
FEATURES             Location/Qualifiers
     source          1..654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..86
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     102..118
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      147..156
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             156..179
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             192..218
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    234..258
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgggtcagcc ctttgtacac cctaagcctc cgcctcctct tcctccatcc gccccgtctc
       61 tcccccttga acctcctcgt tcgaccccgc ctcgatcctc cctttatcca gccctcactc
      121 cttctctagg cgccggaatt agatctgcca ccatggacta caaggatgac gatgacaagc
      181 tcgatggagg atacccatac gatgttccag attacgctgc tcgaggttat caaacaagtt
      241 tgtacaaaaa agttggcatg gccccgcggc tgcagctgga gaaggcggcc tggcgctggg
      301 cggagacggt gcggcccgag gaggtgtcgc aggagcacat cgagaccgct taccgcatct
      361 ggctggagcc ctgcattcgc ggcgtgtgca gacgaaactg caaaggaaat ccgaattgct
      421 tggttggtat tggtgagcat atttggttag gagaaataga tgaaaatagt tttcataaca
      481 tcgatgatcc caactgtgag aggagaaaaa agaactcatt tgtgggcctg actaaccttg
      541 gagccacttg ttatgtcaac acatttcttc aagtgtggtt tctcaacttg gagcttcggc
      601 aggcactcta cttatgtcca agcacttgta gtgactacat gctgggagac ggca
//
