LOCUS       sequence-28388-e         618 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP49
FEATURES             Location/Qualifiers
     source          1..618
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..77
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     93..109
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      138..147
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             147..170
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             183..209
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    225..249
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cctttgtaca ccctaagcct ccgcctcctc ttcctccatc cgccccgtct ctcccccttg
       61 aacctcctcg ttcgaccccg cctcgatcct ccctttatcc agccctcact ccttctctag
      121 gcgccggaat tagatctgcc accatggact acaaggatga cgatgacaag ctcgatggag
      181 gatacccata cgatgttcca gattacgctg ctcgaggtta tcaaacaagt ttgtacaaaa
      241 aagttggcat ggatagatgc aaacatgtag ggcggttacg gctcgcccag gaccactcca
      301 tcctgaaccc tcagaagtgg tgctgcttag agtgtgccac caccgagtcc gtgtgggcct
      361 gcctcaagtg ctcccacgtg gcctgcggcc gctatattga ggaccacgcc ctgaaacact
      421 ttgaggagac gggacacccg ctagccatgg aagtccggga tctctacgtg ttctgttacc
      481 tgtgcaagga ctacgtgctc aatgataacc canaggggga cctgaagctg ctaagaagct
      541 ccctcctggc ggtccggggc cagaaacagg acacgccggt gagacgtggg cggacgctgc
      601 ggnccatggc ttcnggtg
//
