LOCUS       sequence-283887-        2819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1067
FEATURES             Location/Qualifiers
     source          1..2819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2116..2125
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgcagagaga ggctgcgttc agactggggc actgccatcc cctccgcatc
       61 atggggtctg tggaccaaga agagccgaat gcacataagg tcgccagccc accctccgga
      121 cccgcatacc ccgatgatgt cctggactat ggcctcaagc catacagccc ccttgctagt
      181 ctctctggcg agccccccgg ccgattcgga gagccggata gggtagggcc gcagaagttt
      241 ctgagcgcgg ccaagccagc aggggcctcg ggcctgagcc ctcggatcga gatcactccg
      301 tcccacgaac tgatccaggc agtggggccc ctccgcatga gagacgcggg cctcctggtg
      361 gagcagccgc ccctggccgg ggtggccgcc agcccgaggt tcaccctgcc cgtgcccggc
      421 ttcgagggct accgcgagcc gctttgcttg agccccgcta gcagcggctc ctctgccagc
      481 ttcatttctg acaccttctc cccctacacc tcgccctgcg tctcgcccaa taacggcggg
      541 cccgacgacc tgtgtccgca gtttcaaaac atccctgctc attattcccc cagaacctcg
      601 ccaataatgt cacctcgaac cagcctcgcc gaggacagct gcctgggccg ccactcgccc
      661 gtgccccgtc cggcctcccg ctcctcatcg cctggtgcca agcggaggca ttcgtgcgcc
      721 gaggccttgg ttgccctgcc gcccggagcc tcaccccagc gctcccggag cccctcgccg
      781 cagccctcat ctcacgtggc accccaggac cacggctccc cggctgggta cccccctgtg
      841 gctggctctg ccgtgatcat ggatgccctg aacagcctcg ccacggactc gccttgtggg
      901 atccccccca agatgtggaa gaccagccct gacccctcgc cggtgtctgc cgccccatcc
      961 aaggccggcc tgcctcgcca catctacccg gccgtggagt tcctggggcc ctgcgagcag
     1021 ggcgagagga gaaactcggc tccagaatcc atcctgctgg ttccgcccac ttggcccaag
     1081 ccgctggtgc ctgccattcc catctgcagc atcccagtga ctgcatccct ccctccactt
     1141 gagtggccgc tgtccagtca gtcaggctct tacgagctgc ggatcgaggt gcagcccaag
     1201 ccacatcacc gggcccacta tgagacagaa ggcagccgag gggctgtcaa agctccaact
     1261 ggaggccacc ctgtggttca gctccatggc tacatggaaa acaagcctct gggacttcag
     1321 atcttcattg ggacagctga tgagcggatc cttaagccgc acgccttcta ccaggtgcac
     1381 cgaatcacgg ggaaaactgt caccaccacc agctatgaga agatagtggg caacaccaaa
     1441 gtcctggaga tacccttgga gcccaaaaac aacatgaggg caaccatcga ctgtgcgggg
     1501 atcttgaagc ttagaaacgc cgacattgag ctgcggaaag gcgagacgga cattggaaga
     1561 aagaacacgc gggtgagact ggttttccga gttcacatcc cagagtccag tggcagaatc
     1621 gtctctttac agactgcatc taaccccatc gagtgctccc agcgatctgc tcacgagctg
     1681 cccatggttg aaagacaaga cacagacagc tgcctggtct atggcggcca gcaaatgatc
     1741 ctcacggggc agaactttac atccgagtcc aaagttgtgt ttactgagaa gaccacagat
     1801 ggacagcaaa tttgggagat ggaagccacg gtggataagg acaagagcca gcccaacatg
     1861 ctttttgttg agatccctga atatcggaac aagcatatcc gcacacctgt aaaagtgaac
     1921 ttctacgtca tcaatgggaa gagaaaacga agtcagcctc agcactttac ctaccaccca
     1981 gtcccagcca tcaagacgga gcccacggat gaatatgacc ccactctgat ctgcagcccc
     2041 acccatggag gcctggggag ccagccttac tacccccagc acccgatggt ggccgagtcc
     2101 ccctcctgcc tcgtggccac catggctccc tgccagcagt tccgcacggg gctctcatcc
     2161 cctgacgccc gctaccagca acagaaccca gcggccgtac tctaccagcg gagcaagagc
     2221 ctgagcccca gcctgctggg ctatcagcag ccggccctca tggccgcccc gctgtccctt
     2281 gcggacgctc accgctctgt gctggtgcac gccggctccc agggccagag ctcagccctg
     2341 ctccacccct ctccgaccaa ccagcaggcc tcgcctgtga tccactactc acccaccaac
     2401 cagcagctgc gctgcggaag ccaccaggag ttccagcaca tcatgtactg cgagaatttc
     2461 gcaccaggca ccaccagacc tggcccgccc ccggtcagtc aaggtcagag gctgagcccg
     2521 ggttcctacc ccacagtcat tcagcagcag aatgccacga gccaaagagc cgccaaaaac
     2581 ggacccccgg tcagtgacca aaaggaagta ttacctgcgg gggtgaccat taaacaggag
     2641 cagaacttgg accagaccta cttggatgat gttaatgaaa ttatcaggaa ggagttttca
     2701 ggacctcctg ccagaaatca gacgtagtaa tgaactagtc cgtaacttga aagtatttcg
     2761 atttcttggc tttatatatc ttgtggaaag gacgactaca ggacaatcaa agcgtgtgc
//
