LOCUS       sequence-28389-e         682 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP50
FEATURES             Location/Qualifiers
     source          1..682
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     69..91
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     107..123
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      152..161
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             161..184
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             197..223
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    239..263
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctccctggnn cngccctttg tacaccctaa gcctccgcct cctcttcctc catccgcccc
       61 gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt atccagccct
      121 cactccttct ctaggcgccg gaattagatc tgccaccatg gactacaagg atgacgatga
      181 caagctcgat ggaggatacc catacgatgt tccagattac gctgctcgag gttatcaaac
      241 aagtttgtac aaaaaagcag aagggccgtc aaggcccacc atgacttctc agccgtctct
      301 ccctgcagat gacttcgata tctaccacgt ccttgcagag tgcacagatt actatgatac
      361 ccttccagtt aaggaggctg atgggaacca gccccatttt cagggtgtca ctggcttgtg
      421 gaacttgggc aacacatgct gcgtgaatgc catctcacag tgtctctgca gcatcttgcc
      481 gctggtggaa tactttctca ccgggaagta tatcaccgct ctgcaaaagt ttcttcttcc
      541 aagcgattgc agtgaagttg ccactgcttt tgcctatctg atgacagaca tgtggctggg
      601 agactcagac tgtgtctcac cagaaatatt ctggtcagct cttggcaacc tctacccagc
      661 atttacgaaa aagatgcaac aa
//
