LOCUS       sequence-283893-        2063 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1083
FEATURES             Location/Qualifiers
     source          1..2063
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagacta ttcaacagtg cctcccccct cttctggctc agctggtggc
       61 ggtggtggcg gcggtggtgg tggaggagtt aacgacgctt tcaaagatgc actgcagaga
      121 gcccggcaga ttgcagcaaa aattggaggt gatgcaggga catcactgaa ttcaaatgac
      181 tatggttatg ggggacaaaa aagaccttta gaagatggag atcaaccaga tgctaagaaa
      241 gttgctcctc aaaatgactc ttttggaaca cagttaccac cgatgcatca gcagcaaaga
      301 tctgtaatga cagaagaata caaagttcca gatggaatgg ttggattcat aattggcaga
      361 ggaggtgaac agatctcacg catacaacag gaatctggat gcaaaataca gatagctcct
      421 gacagtggtg gccttccaga aaggtcctgt atgttaactg gaacacctga atctgtccag
      481 tcagcaaaac ggttactgga ccagattgtt gaaaaaggaa gaccagctcc tggcttccat
      541 catggcgatg gaccgggaaa tgcagttcaa gaaatcatga ttccagctag caaggcagga
      601 ttagtcattg gaaaaggggg agaaactatt aaacagcttc aggaacgggc tggagttaaa
      661 atggttatga ttcaagacgg gccgcagaac actggtgctg acaaacctct taggattaca
      721 ggagacccat ataaagttca acaagccaag gaaatggtgt tagagttaat tcgtgatcaa
      781 ggcggtttca gagaagttcg gaatgagtat gggtcaagaa taggaggaaa tgaagggata
      841 gatgtcccca ttccaagatt tgctgttggc attgtaatag gaagaaatgg agagatgatc
      901 aaaaaaatac aaaatgatgc tggtgttcgc attcagttta agccagatga tgggacaaca
      961 cccgaaagga tagcacaaat aacaggacct ccagaccgat gtcaacatgc tgcagaaatt
     1021 attacagacc ttcttcgaag tgttcaggct ggtaatcctg gtggacctgg acctggtggt
     1081 cgaggaagag gtagaggtca aggcaactgg aacatgggac cacctggtgg actacaggaa
     1141 tttaatttta ttgtgccaac tgggaaaact ggattaataa taggaaaagg aggtgaaacc
     1201 ataaaaagca taagccagca gtctggtgca agaatagaac ttcagagaaa tcctccacca
     1261 aatgcagatc ctaatatgaa gttatttaca attcgtggca ctccacaaca gatagactat
     1321 gctcggcaac tcatagaaga aaagattggt ggcccagtaa atcctttagg gccacctgta
     1381 ccccatgggc cccatggtgt cccaggcccc catggacctc ctgggcctcc agggcctgga
     1441 actccaatgg gaccatacaa ccctgcacct tataatcctg gaccaccagg cccggctcct
     1501 catggtcctc cagccccata tgctccccag ggatggggaa atgcatatcc acactggcag
     1561 cagcaggctc ctcctgatcc agctaaggca ggaacggatc caaattcagc agcttgggct
     1621 gcttattacg ctcactatta tcaacagcaa gcacagccac caccagcagc ccctgcaggt
     1681 gcaccaacta caactcaaac taatggacaa ggagatcagc agaatccagc cccagctgga
     1741 caggttgatt ataccaaggc ttgggaagag tactacaaga aaatgggtca ggcagttcct
     1801 gctccgactg gggctcctcc aggtggtcag ccagattata gtgcagcctg ggctgagtat
     1861 tatagacaac aagcagccta ttatgcccag acaagtcccc agggaatgcc acagcatcct
     1921 ccagcacctc agtgcaggtt tgatccagcc agtatagaac tagctctgta gtaatgaact
     1981 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgag
     2041 gtggtaacgg catcttaatg cca
//
