LOCUS       sequence-283896-        1280 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1095
FEATURES             Location/Qualifiers
     source          1..1280
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaagaaca gcagccggaa cctaaaagtc agcgcgactc ggccctcggc
       61 gcggcggcgg cggcgactcc gggcggcctc agcctgagcc tcagtccggg cgccagcggc
      121 agcagcggca gcggcagcga tggagacagc gtgccggtgt ccccgcagcc tgcgcccccc
      181 tcgccgcccg cggcgccttg cctgccgccc ctggcccacc acccgcacct ccccccacac
      241 cccccgcccc cgccgcctca gcatctcgcg gcgcctgctc accagccgca gccagcggcc
      301 cagctgcacc gcaccaccaa ctttttcatc gacaacatcc tgaggccgga cttcggctgc
      361 aaaaaggagc agccgccacc gcagcttctg gtggctgcgg cggccagagg aggcgcagga
      421 ggaggaggcc gggtcgagcg tgacagaggc cagactgccg caggtagaga ccctgtccac
      481 ccgttgggca cccgggcgcc aggcgctgcc tcgctcctgt gcgccccgga cgcgaactgt
      541 ggcccacccg acggctccca gccagccgcc gccggcgcgg gcgcgtctaa agctgggaac
      601 ccggctgcgg cggcggcggc ggccgcggcg gcagtggcgg cggcggcggc ggccgcagca
      661 gccaagccct cggacaccgg tggcggcggc agtggaggcg gcgcggggag ccccggagcg
      721 cagggcacca aatacccgga gcacggcaac ccggctatcc tacttatggg ctcagccaac
      781 ggcgggcccg tggtcaaaac tgactcgcag cagcctctcg tatggcccgc ctgggtgtac
      841 tgcacacgtt attcggatcg tccatcctcc ggtccgcgca ccaggaagct gaagaagaag
      901 aagaacgaga aggaggacaa gcggccgcgg accgcgttca cggccgagca gctgcagaga
      961 ctcaaggcgg agttccaggc aaaccgctac atcacggagc agcggcggca gaccctggcc
     1021 caggaactca gcctcaacga gtcccagatc aagatctggt tccagaacaa gcgcgccaag
     1081 atcaagaaag ccacaggcat caagaacggc ctggcgctgc acctcatggc ccagggactg
     1141 tacaaccact ccaccaccac ggtccaggac aaagacgaga gcgagtagta atgaactagt
     1201 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgattgt
     1261 ggtgtggcca aactgcccac
//
