LOCUS       sequence-28391-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP5
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     58..80
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     96..112
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      141..150
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             150..173
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             186..212
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    228..252
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agccctttgt acnccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
       61 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
      121 taggcgccgg aattagatct gccaccatgg actacaagga tgacgatgac aagctcgatg
      181 gaggataccc atacgatgtt ccagattacg ctgctcgagg ttatcaaaca agtttgtaca
      241 aaaaagttgg catggcggag ctgagtgagg aggcgctgct gtcagtatta ccgacgatcc
      301 gggtccctaa ggctggagac cgggtccaca aagacgagtg cgccttctcc ttcgacacgc
      361 cggagtctga ggggggcctc tacatctgta tgaacacgtt tctgggcttt gggaaacagt
      421 atgtggagag acatttcaat aagaccggcc agcgagtcta cttgcacctc cggcggaccc
      481 ggcgcccgaa agaggaggac cctgctacag gcactggaga cccaccccgg aagaagccca
      541 cgcggctggc tattggtgtt gaaggcggat ttgaccttag cgaggagaag tttgaattag
      601 acgaggatgt gaagattgtc attttgccag attacctgga gattgcccgg gatggactgg
      661 ggggactgcc tgacattgtc agagatcggg tgaccagtgc agtggaggcc ctactgtcgg
      721 ccgactcagc ctcccgcaag caggangtgc aggcatggga tggggaagta cggcaggtgt
      781 ctaagcatgc cttcagcctc aagcagttgg acaaccctgc tcgaatccct ccctgtggct
      841 ggaagtgntc caagtgtgac atganagaga acctg
//
