LOCUS       sequence-28393-e         582 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-VCPIP1
FEATURES             Location/Qualifiers
     source          1..582
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      42..51
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             51..74
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             87..113
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    129..153
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atccagccct cactccttct ctaggcgccg gaattagatc tgccaccatg gactacaagg
       61 atgacgatga caagctcgat ggaggatacc catacgatgt tccagattac gctgctcgag
      121 gttatcaaac aagtttgtac aaaaaagttg gcatgtctca gccgccgccg ccgccgcctc
      181 cgttgccgcc gccacctcct ccccctgagg ctccacagac tccgtcgtcc ttggcgtcgg
      241 cggctgcttc gggggggctt ttgaagcgga gagaccggag aatcctttcc gggagctgcc
      301 cggatccgaa gtgtcaggcg cgtctatttt tcccggcctc cggttctgtc agcatcgagt
      361 gtaccgagtg cggccagcgg cacgagcagc aacagctgct gggggttgag gaggtgaccg
      421 acccggacgt agtgctacac aacctgctgc ggaacgcgct gctcggggtt acgggggcac
      481 ccaagaagaa cacggaactg gtaaaggtga ngggcctttc cnactatcac tgcaaattgt
      541 tgtcgcccat attagctcgc tatggaatgg acaaacagac ag
//
