LOCUS       sequence-283932-        1415 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1173
FEATURES             Location/Qualifiers
     source          1..1415
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagcggc cactctgagg acgccaactc agggcactgt gacctttgaa
       61 gatgtggccg tacacttctc ctgggaggaa tggggtctcc ttgatgaggc tcaaagatgc
      121 ctgtaccgtg atgtgatgct ggagaacttg gctcttttaa cctctctaga tgttcatcat
      181 cagaagcagc accttggaga aaaacatttc agaagcaatg tgggcagagc cttgtttgtg
      241 aagacctgca cattccatgt gtcaggggag ccttccacct gcagagaagt tgggaaggac
      301 ttcctggcca agttgggatt tctccatcaa caggctgctc acactgggga gcaatcaaat
      361 agcaaaagcg acggtggggc catcagtcac agaggaaaaa ctcattacaa ctgtggagaa
      421 cacacaaaag cattcagcgg taaacacaca cttgttcagc agcagagaac cctcactaca
      481 gaaagatgtt acatatgcag tgaatgtggg aaatccttta gcaaaagcta cagtctcaat
      541 gaccattgga gacttcacac tggagaaaag ccttatgaat gtcgagagtg tgggaagtcc
      601 tttaggcaaa gctctagtct cattcagcac cggagagttc acactgcagt acgacctcat
      661 gaatgtgatg aatgtggaaa attatttagc aacaagtcta acctcattaa acatcggaga
      721 gttcacactg gggaaaggcc atatgagtgc agtgaatgtg ggaaatcctt tagccaaagg
      781 tctgcactcc ttcaacatcg gggagttcac actggggaga ggccttatga gtgcagtgaa
      841 tgtgggaagt tttttacata tcattccagt ctcataaaac accagaaagt tcacagtgga
      901 tccaggcctt atgagtgcag cgaatgtggg aagtcattta gccaaaactc tagcctcatt
      961 gaacaccata gggttcacac tggagaaagg ccttataagt gcagcgaatg tgggaaatcc
     1021 tttagccaaa ggtctgcact ccttcaacat cggggagttc acactgggga gaggccttat
     1081 gagtgcagtg aatgtgggaa gttctttccc tacagctcca gtctccgaaa acaccagaga
     1141 gttcacactg gatcaagacc ctatgagtgc agtgaatgtg ggaaatcctt tactcaaaat
     1201 tccggcctca ttaagcacag gagggttcac actggggaga agccttatga gtgcacggaa
     1261 tgtgggaaat cctttagcca taactccagc cttattaaac atcagagaat tcatagtcga
     1321 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1381 ggaaaggacg acaggattcc cttgatacta tacag
//
