LOCUS       sequence-283935-        1799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1183
FEATURES             Location/Qualifiers
     source          1..1799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggactc ggactcggcg gcaaagcagc tgggcctggc tgaggcggcg
       61 gcggtggcgg ccgcggccgc tgtggcggcg gcggccgcgg ccgcggcagg aggcgaggcg
      121 gaggagccgg tgctgagcag ggacgaggac tcggaggagg acgcagactc ggaggcggag
      181 cgggagacgc cgcgggtcac ggcagtggcg gtgatggcgg cggagcccgg gcacatggac
      241 atgggcgccg aggccctgcc cggccccgac gaggccgccg ctgccgcagc cttcgcagag
      301 gtgaccacag tgacagtggc caacgtgggg gctgctgcag acaatgtctt caccacgtct
      361 gtggcgaacg cggcatccat ctcaggacat gttctgtctg gtaggacggc ccttcagatc
      421 ggggacagcc tgaacaccga aaaagcgaca ctgattgtcg tccacacaga tgggagcatc
      481 gtggagacca ccgggctgaa aggcccggca gctcccctca ccccaggtcc tcagtctcct
      541 ccaacccctc tggctcccgg ccaagaaaaa ggtggaacta aatacaactg ggacccttct
      601 gtgtacgaca gtgagctgcc cgtacggtgc cggaacatca gcggcactct gtacaagaac
      661 aggctcggct caggcggccg gggacggtgc atcaagcagg gggagaactg gtacagtccc
      721 accgagtttg aggccatggc aggaagagcc agcagtaagg actggaaaag aagcattcgc
      781 tacgcgggcc gacccttgca gtgcctcatc caggatggga tcttaaaccc tcacgctgcc
      841 tcttgcacct gtgctgcctg ctgcgacgac atgaccttaa gtggcccagt caggcttttt
      901 gtgccttaca aaaggcgcaa gaaggagaat gaactgccca caactcccgt gaagaaggac
      961 tcccccaaga acatcacatt gcttccagcc accgcggcta ccaccttcac cgtgaccccc
     1021 tcgggacaga tcacgacctc gggggcactg acctttgacc gagcgtccac ggtagaggcc
     1081 actgctgtca tatcagagag tccggcccag ggcgacgtct tcgcaggggc cacagtccaa
     1141 gaggccagcg tgcagccccc atgcagggcc agccaccctg agcctcacta ccccggctat
     1201 caggacagct gccagatcgc accgtttcca gaagctgcgt tgccaacgtc acatcccaaa
     1261 atagtgttga catccctgcc tgcgctggcg gtcccacccc cgactcccac caaagcggca
     1321 cctcccgcgt tggtcaatgg gctggagctg tcagagccgc ggagctggct gtacctagaa
     1381 gagatggtca actccttgct caacacagcg cagcagctga agacgctgtt tgagcaagcc
     1441 aagcatgcca gcacctaccg agaagctgcc acaaaccagg ccaagatcca cgctgacgca
     1501 gagcggaagg agcagtcctg cgttaactgc ggccgggagg ctatgagcga gtgcaccggc
     1561 tgccacaagg tcaactactg ctccaccttc tgccaacgca aggactggaa ggatcaccag
     1621 cacatatgcg gccagtcagc agctgtcacc gtccaggcag acgaagtcca cgtggctgaa
     1681 agcgtgatgg agaaggtgac cgtgtagtaa tgaactagtc cgtaacttga aagtatttcg
     1741 atttcttggc tttatatatc ttgtggaaag gacgacctgg gcaattcagg agatccctg
//
