LOCUS       sequence-283938-        2225 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1204
FEATURES             Location/Qualifiers
     source          1..2225
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggagaaaa aaatggcgat gcaaaaactt tctggatgga gctagaagat
       61 gatggaaaag tggacttcat ttttgaacaa gtacaaaatg tgctgcagtc actgaaacaa
      121 aagatcaaag atgggtctgc caccaataaa gaatacatcc aagcaatgat tctagtgaat
      181 gaagcaacta taattaacag ttcaacatca ataaaggatc ctatgcctgt gactcagaag
      241 gaacaggaaa acaaatccaa tgcatttccc tctacatcat gtgaaaactc ctttccagaa
      301 gactgtacat ttctaacaac agaaaataag gaaattctct ctcttgaaga taaagttgta
      361 gactttagag aaaaagactc atcttcgaat ttatcttacc aaagtcatga ctgctctggt
      421 gcttgtctga tgaaaatgcc actgaacttg aagggagaaa accctctgca gctgccaatc
      481 aaatgtcact tccaaagacg acatgcaaag acaaactctc attcttcagc actccacgtg
      541 agttataaaa ccccttgtgg aaggagtcta cgaaacgtgg aggaagtttt tcgttacctg
      601 cttgagacag agtgtaactt tttatttaca gataactttt ctttcaatac ctatgttcag
      661 ttggctcgga attacccaaa gcaaaaagaa gttgtttctg atgtggatat tagcaatgga
      721 gtggaatcag tgcccatttc tttctgtaat gaaattgaca gtagaaagct cccacagttt
      781 aagtacagaa agactgtgtg gcctcgagca tataatctaa ccaacttttc cagcatgttt
      841 actgattcct gtgactgctc tgagggctgc atagacataa caaaatgtgc atgtcttcaa
      901 ctgacagcaa ggaatgccaa aacttccccc ttgtcaagtg acaaaataac cactggatat
      961 aaatataaaa gactacagag acagattcct actggcattt atgaatgcag ccttttgtgc
     1021 aaatgtaatc gacaattgtg tcaaaaccga gttgtccaac atggtcctca agtgaggtta
     1081 caggtgttca aaactgagca gaagggatgg ggtgtacgct gtctagatga cattgacaga
     1141 gggacatttg tttgcattta ttcaggaaga ttactaagca gagctaacac tgaaaaatct
     1201 tatggtattg atgaaaacgg gagagatgag aatactatga aaaatatatt ttcaaaaaag
     1261 aggaaattag aagttgcatg ttcagattgt gaagttgaag ttctcccatt aggattggaa
     1321 acacatccta gaactgctaa aactgagaaa tgtccaccaa agttcagtaa taatcccaag
     1381 gagcttactg tggaaacgaa atatgataat atttcaagaa ttcaatatca ttcagttatt
     1441 agagatcctg aatccaagac agccattttt caacacaatg ggaaaaaaat ggaatttgtt
     1501 tcctcggagt ctgtcactcc agaagataat gatggattta aaccaccccg agagcatctg
     1561 aactctaaaa ccaagggagc acaaaaggac tcaagttcaa accatgttga tgagtttgaa
     1621 gataatctgc tgattgaatc agatgtgata gatataacta aatatagaga agaaactcca
     1681 ccaaggagca gatgtaacca ggcgaccaca ttggataatc agaatattaa aaaggcaatt
     1741 gaggttcaaa ttcagaaacc ccaagaggga cgatctacag catgtcaaag acagcaggta
     1801 ttttgtgatg aagagttgct aagtgaaacc aagaatactt catctgattc tctaacaaag
     1861 ttcaataaag ggaatgtgtt tttattggat gccacaaaag aaggaaatgt cggccgcttc
     1921 cttaatcata gttgttgccc aaatctcttg gtacagaatg tttttgtaga aacacacaac
     1981 aggaattttc cattggtggc attcttcacc aacaggtatg tgaaagcaag aacagagcta
     2041 acatgggatt atggctatga agctgggact gtgcctgaga aggaaatctt ctgccaatgt
     2101 ggggttaata aatgtagaaa aaaaatatta tagtaatgaa ctagtccgta acttgaaagt
     2161 atttcgattt cttggcttta tatatcttgt ggaaaggacg acaactgatg gagatgtcaa
     2221 tacag
//
