LOCUS       sequence-28394-e         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-DUB3
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..25
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    28..46
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     56..80
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      153..162
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             162..185
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             198..224
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    240..264
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gagctctccc tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc
       61 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
      121 gacaccggga ccgatccagc ctccggggat ctgccaccat ggactacaag gatgacgatg
      181 acaagctcga tggaggatac ccatacgatg ttccagatta cgctgctcga ggttatcaaa
      241 caagtttgta caaaaaagtt ggcatggagg acgactcact ctacttggga ggtgagtggc
      301 agttcaacca cttttcaaaa ctcacatctt ctcggcccga tgcagctttt gctgaaatcc
      361 agcggacttc tctccctgag aagtcaccac tctcatgtga gacccgtgtc gacctctgtg
      421 atgatttggc tcctgtggca agacagcttg ctcccaggga gaagcttcct ctgagtagca
      481 ggagacctgc tgcggtgggg gctgggctcc agaatatggg aaatacctgc tacgtgaacg
      541 cttccttgca gtgcctgaca tacacaccgc cccttgccaa ctacatgctg tcccgggagc
      601 actctcaaac gtgtcatcgt cacaagggct gcatgctctg tactatgcaa gctcacatca
      661 cacgggccct ccacaatcct ggccacgttc atccagccct cacaggcatt ggctgctggc
      721 ttccatagag gcaagcagga agatgcccat gaatttctca tgttcactg
//
