LOCUS       sequence-283941-        1325 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1211
FEATURES             Location/Qualifiers
     source          1..1325
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagagccg aaaggacatg gttgtgtttc tggatggggg tcagcttggc
       61 actctggttg gcaagagagt ctcaaatttg tccgaagccg tgggcagccc gctgccggag
      121 ccgcccgaga aaatggtgcc ccgtggttgc ctgagccctc gggccgtccc tccggccacc
      181 cgggagcgcg gcgggggagg cccggaggag gagccggtag atggactcgc aggcagcgcg
      241 gcggggccgg gcgccgagcc ccaggtagct ggggcggcca tgctcggccc aggacccccg
      301 gccccctcag tcgacagcct ctccggacag gggcaaccca gtagctcgga caccgagtcg
      361 gatttctatg aagaaatcga ggtgagctgc accccggact gcgccaccgg gaacgccgag
      421 taccagcaca gcaaagggtc cggctccgag gcgctggtcg gcagtccgaa cggagggagc
      481 gagaccccca agagcaacgg cggcagtggt gggggcggct cgcaaggcac cctggcgtgc
      541 agcgccagtg accagatgcg tcgttaccgc accgccttca cccgagagca gattgcgcgg
      601 ctggagaagg aattctaccg ggagaactac gtatccaggc cgcggagatg tgagctggcg
      661 gccgccctaa acctgccgga aaccaccatc aaggtgtggt tccagaaccg gcgcatgaag
      721 gacaagcggc agcgcctggc catgacgtgg ccgcacccgg cggaccccgc cttctacact
      781 tacatgatga gccatgcggc ggccgcgggc ggcctgccct accccttccc atcgcacctg
      841 cccctgccct actactcgcc ggtgggcctg ggcgccgcat ccgccgcctc cgccgccgcc
      901 tcgcccttca gcggctcgct gcgcccgctc gacacgttcc gcgtgctgtc gcagccctac
      961 ccgcggcccg aactgctgtg cgccttccgc cacccgccgc tctaccccgg gcccgcgcac
     1021 ggactgggcg cctctgccgg cggcccctgc tcctgcctcg cctgtcacag cggcccggcc
     1081 aacgggctgg cgccccgggc tgccgccgcc tcggacttca cctgtgcctc cacctcccgc
     1141 tcggactcct tcctcacctt cgcgccctcg gtgctcagca aggcctcctc cgtcgcgctg
     1201 gaccagaggg aggaggtgcc cctcactaga tagtaatgaa ctagtccgta acttgaaagt
     1261 atttcgattt cttggcttta tatatcttgt ggaaaggacg aaccaaatcc cgttgggtgc
     1321 gagag
//
