LOCUS       sequence-283944-         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1219
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacagcgc ggccgccgcc ttcgccctgg acaagccggc gctgggcccg
       61 gggccgccgc cgcctccacc cgcgctgggg cccggcgact gcgcccaggc gcgcaagaac
      121 ttctcggtga gccacctcct ggacctggaa gaggtggcgg cggccgggcg gctggcggcg
      181 cgccccgggg ccagggccga ggcgcgggag ggcgcagcac gggagccgtc cgggggcagc
      241 agcggcagcg aggcggcgcc gcaggatggt gagtgtccca gcccggggcg cggtagcgcc
      301 gccaagcgga agaagaagca gcggcggaac cgcaccacgt tcaacagcag ccaactgcag
      361 gcgctggagc gcgtgttcga gcgcacgcac taccccgacg cctttgtgcg cgaggagctt
      421 gcccggcgcg tcaacctcag cgaggcgcgc gttcaggtct ggtttcagaa ccgccgcgcc
      481 aagttccgca ggaatgaaag ggccatgctg gccagccgct ctgcctcgct gctcaagtcc
      541 tacagccagg aggccgccat cgagcagccc gtggctcccc ggcccaccgc cctgagtcca
      601 gattatctct cctggacagc ctcgtccccc tacagcacag tgccacccta cagccctggg
      661 agctcaggcc ccgcaacccc aggggtcaac atggccaaca gcatcgccag cctccgtctc
      721 aaggccaagg agttcagcct gcaccacagc caggtgccta cggtgaacta gtaatgaact
      781 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgat
      841 ttgcagcttg accacacgtc tgc
//
