LOCUS       sequence-283950-        3593 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1229
FEATURES             Location/Qualifiers
     source          1..3593
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(233..244)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 accgccacca tgaaccgtga agaccggaat gtgctgcgta tgaaagaacg ggaaaggcgg
       61 aatcaggaaa ttcagcaggg cgaagacgcc ttcccaccta gctctcctct ctttgcagag
      121 ccatacaaag ttactagcaa agaagataag ttatcaagtc gtattcagag tatgcttgga
      181 aactacgatg aaatgaagga tttcatagga gacagatcta taccaaagct tgttgcaatt
      241 cccaagccta cagtaccacc atcagcagat gaaaaatcta acccaaattt ctttgaacag
      301 agacatggag gctctcatca gagtagcaaa tggactccag taggacccgc acccagcact
      361 tctcagtctc agaaacggtc ctcaggctta cagagtggac atagtagcca gcggaccagc
      421 gcaggtagca gtagtggcac taacagtagt ggtcagaggc acgaccgtga gtcatataac
      481 aatagtggga gcagtagccg gaaaaaaggc cagcatggat cagaacactc caaatcacgt
      541 tcttccagcc ctggaaaacc ccaggctgtt tcttcattaa actctagtca ttccaggtct
      601 catgggaatg atcaccatag caaggaacat caacgctcca aatcacctcg ggaccctgat
      661 gcaaactggg attctccttc ccgtgtacct ttttcaagtg ggcagcactc aactcaatct
      721 ttcccaccct cattgatgtc aaagtccaat tcaatgttac agaaacccac tgcctatgtg
      781 cggcccatgg acggacagga gtccatggaa ccaaagctgt cctctgagca ctacagcagc
      841 caatcccatg gcaacagcat gactgagctg aagcccagca gcaaagcaca tctcaccaag
      901 ctgaaaatac cttcccaacc actggatgca tcagcttctg gtgatgtgag ctgtgtggat
      961 gaaatcctaa aagagatgac gcattcatgg cctccccctc taacggctat tcatacacca
     1021 tgcaaaacag aaccttccaa atttcctttt ccaactaagg agtctcagca gtccaatttt
     1081 ggcactggag aacaaaaaag atataatcct tctaaaactt caaatgggca ccagtctaaa
     1141 tctatgttaa aagatgactt aaaactaagc agcagtgaag acagtgatgg ggaacaggat
     1201 tgtgataaga caatgccgag gagtacacca ggaagtaact ctgaaccttc acaccataat
     1261 agtgaaggag cagataactc cagggatgat tctagtagcc acagtggatc tgaaagcagc
     1321 tctggatctg actcagagag tgaaagtagt tccagtgaca gtgaggcaaa tgagccatcc
     1381 cagagtgcat ctcccgagcc tgaacccccg ccaacaaaca aatggcaact tgataattgg
     1441 ctgaataaag tgaacccaca taaagtgtca cccgcctctt cagtggacag taacatccca
     1501 tcatctcaag gctacaaaaa ggaaggccga gagcagggca ctgggaatag ctacactgat
     1561 acaagtggac ctaaagaaac gagttccgct actccgggac gagactccaa aaccatccaa
     1621 aagggatcag aaagtgggcg tgggaggcag aaatctcctg cacagagtga cagcacaaca
     1681 cagagaagaa ctgtaggcaa aaaacaaccc aaaaaggctg agaaggcagc tgctgaagag
     1741 cctcgtggag gcctgaagat agaaagtgaa acccctgtag acttggctag cagcatgccc
     1801 tccagcagac acaaagcagc caccaaaggc tcaaggaaac ccaatataaa gaaggagtct
     1861 aagtcttccc ctcgacctac agcagagaaa aagaaatata agtcaacaag taaatcttcc
     1921 cagaaatcaa gggaaatcat agaaacagat acctcatcct cagattcaga tgaaagtgag
     1981 agccttcctc cttcctcaca aactcctaag taccccgaga gcaataggac tcctgttaaa
     2041 ccctcctcag tggaggaaga agatagcttt tttcggcaac gaatgttctc tcctatggaa
     2101 gagaaggaac ttctttcacc cctcagtgag cctgatgaca ggtacccact tattgtgaag
     2161 attgacctga atcttttgac tagaatacca ggaaagcctt acaaagaaac agagccgccc
     2221 aagggggaaa agaaaaatgt gccagaaaag cacacgagag aggctcagaa acaagcctca
     2281 gaaaaagttt ccaacaaagg caagaggaag cataagaatg aagatgataa ccgagccagt
     2341 gagagcaaga aacccaaaac ggaggacaag aattcagcag gccataagcc atccagcaac
     2401 agagagtcat ctaagcagag tgctgcaaaa gaaaaggatt tgttgccttc tcccgctggg
     2461 cctgttcctt caaaagatcc aaaaacagag catggctctc ggaagaggac tattagtcag
     2521 tcttcttcct taaagtcaag cagtaacagc aacaaggaga cgagtggcag cagcaaaaac
     2581 agttcctcca catcaaagca gaagaagacc gaagggaaga cttccagtag ctccaaggag
     2641 gttaaggaaa aggctccaag tagctcctct aactgtcctc catctgcacc aactcttgat
     2701 tcttctaagc ctcggagaac aaagcttgtc tttgatgaca gaaattattc agcagaccat
     2761 tatttacaag aagcaaaaaa gctaaagcac aatgcagatg cattgtctga taggtttgag
     2821 aaagctgtat actatcttga tgctgtggta tctttcattg aatgtgggaa tgcattagag
     2881 aagaatgctc aggaatccaa atccccattc cctatgtatt cagagacggt ggatctcatc
     2941 aaatacacta tgaagctaaa gaattacttg gcaccagatg ctacagctgc agataaacga
     3001 ctcacagtac tttgcctgcg atgcgagtct ttgctgtacc tgaggctgtt caaactgaag
     3061 aaggaaaatg ctctgaagta ctcaaagaca ctgacagagc acctgaagaa ttcttataat
     3121 aattctcaag caccatcgcc tggcttggga agcaaagctg tggggatgcc ttcccctgtt
     3181 tctccaaagc tgtcaccagg caattcagga aattattcat ctggggccag tagtgcttct
     3241 gcaagtggtt cttcagtgac cattccacag aagatccacc agatggcagc cagctatgtt
     3301 caggtcacat ccaacttcct ctatgccacc gaaatttggg accaagctga acagctttcc
     3361 aaagagcaaa aagaattctt tgctgaactg gataaagtaa tgggccctct catctttaat
     3421 gcaagcatca tgacagatct agttcgttat acccggcagg gactgcactg gcttcgccag
     3481 gatgccaagt tgatatctta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     3541 tggctttata tatcttgtgg aaaggacgat ttccttcaca ggcgcttctt ata
//
