LOCUS       sequence-28396-e         486 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP16
FEATURES             Location/Qualifiers
     source          1..486
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    14..32
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     42..66
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      139..148
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             148..171
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             184..210
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    226..250
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agtgatagag atctccctat cagtgataga gatcgtcgac gagctcgttt agtgaaccgt
       61 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga
      121 tccagcctcc ggggatctgc caccatggac tacaaggatg acgatgacaa gctcgatgga
      181 ggatacccat acgatgttcc agattacgct gctcgaggtt atcaaacaag tttgtacaaa
      241 aaagttggca tgggaaagaa acggactaag ggaaaaactg ttccaatcga tgattcctct
      301 gaaactttag aacctgtgtg cagacacatt agaaaaggat tggaacaagg taatttgaaa
      361 aaggctttan tgnatgtgga atggaatatc tgccaagact gtaagactga caataaagtg
      421 aaagataaag ctgaagaaga aacagaagaa aagccttcag tttggctgtg tctnanatgt
      481 gnccat
//
