LOCUS       sequence-283968-        2822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1288
FEATURES             Location/Qualifiers
     source          1..2822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgcacga atccgagagg agctctcgtc tcggggtgcc ctgcggggag
       61 ccggcagagc tcggaggtga tgctagcgag gaggatcacc cccaagtctg tgccaagtgc
      121 tgcgcacaat tcactgaccc aactgaattc ctcgcccacc agaacgcatg ttctactgac
      181 cctcctgtaa tggtgataat tgggggccag gagaacccca acaactcttc ggcctcctct
      241 gaaccccggc ctgagggtca caataatcct caggtcatgg acacagagca tagcaacccc
      301 ccagattctg ggtcctccgt gcccacggat cccacctggg gcccagagag gagaggagag
      361 gagtcttcag ggcatttcct ggtcgctgcc acaggggcag aaacgcccaa gcaggccttc
      421 ttccaccttt accacccact ggggtcacag catcctttct ctgctggagg ggttgggcga
      481 agccacaaac ccacccctgc cccttcccca gccttgccag gcagcacaga tcagctgatt
      541 gcctcgcctc atctggcatt cccaagcacc acgggactac tggcagcaca gtgtcttggg
      601 gcagcccgag gccttgaggc cactgcctcc ccagggctcc tgaagccaaa gaatggaagt
      661 ggtgagctga gctacggaga agtgatgggt cccttggaga agcctggtgg aaggcacaaa
      721 tgccgcttct gtgccaaagt atttggcagt gacagtgccc tgcagatcca ccttcgttcc
      781 cacacgggtg agaggcccta taagtgcaat gtctgtggaa accgttttac cacccgtggc
      841 aacctcaaag tgcatttcca ccggcatcgt gagaagtacc cacatgtgca gatgaaccca
      901 cacccagtac cagagcacct agactatgtc attaccagca gtggcttgcc ttatggtatg
      961 tccgtgccac cagagaaggc cgaggaggag gcagccactc caggtggagg ggttgagcgc
     1021 aagcctctgg tggcctccac aacagcactc agtgccacag agagcctgac tctgctctcc
     1081 accagtgcag gcacagccac ggctccagga ctccctgctt tcaataagtt tgtgctcatg
     1141 aaagcagtgg aacccaagaa taaagctgat gaaaacaccc ccccagggag tgagggctca
     1201 gccatcagtg gagtggcaga aagtagcacg gcaactcgca tgcaactaag taagttggtg
     1261 acttcactac caagctgggc actgcttacc aaccacttca agtccactgg cagcttcccc
     1321 ttcccctatg tgctagagcc cttgggggcc tcaccctctg agacatcaaa gctgcagcaa
     1381 ctggtagaaa agattgaccg gcaaggagct gtggcggtga cctcagctgc ctcaggagcc
     1441 cccaccacct ctgcccctgc accttcatcc tcagcctctt ctggacctaa ccagtgtgtc
     1501 atctgtctcc gagtgcttag ctgtcctcgg gccctacgcc ttcattatgg ccaacatgga
     1561 ggtgagaggc ccttcaaatg caaagtgtgt ggcagagcct tctccaccag gggtaatctg
     1621 cgtgcacatt tcgtgggcca caaggccagt ccagctgccc gggcacagaa ttcctgcccc
     1681 atctgccaga agaagttcac caatgctgtc actctgcagc agcatgtccg gatgcacctg
     1741 gggggccaga tccccaacgg tggtactgca ctccctgaag gtggaggagc tgctcaggag
     1801 aatggctccg agcaatctac agtctccggg gcacggagtt tcccccagca gcagtcccag
     1861 cagccatcac cggaagagga gttgtctgag gaggaggaag aggaggatga ggaagaagag
     1921 gaagatgtga ctgatgaaga ttccctggca gggagaggct cagagagtgg aggtgagaag
     1981 gcaatatcag tgagaggtga ttcagaagag gcatctgggg cagaggagga ggtggggaca
     2041 gtggcggcag cagccacagc tgggaaggag atggacagta atgagaaaac tactcaacag
     2101 tcttctttgc caccaccacc accacctgac agcctggatc agcctcagcc aatggagcag
     2161 ggaagcagtg gtgttttagg aggcaaggaa gaggggggca aaccggagag aagctcaagt
     2221 ccggcatcag cactcacccc agaaggggaa gccaccagcg tgaccttggt agaggagctg
     2281 agcctgcagg aggcaatgag aaaggagcca ggagagagca gcagcagaaa ggcctgcgaa
     2341 gtgtgtggcc aggcctttcc ctcccaggca gctctggagg agcatcagaa gacccacccc
     2401 aaggaggggc cgctcttcac ttgtgttttc tgcaggcagg gctttcttga gcgggctacc
     2461 ctcaagaagc atatgctcct ggcacaccac cagaaccagt atgtggcatt cctgtcaaat
     2521 ggcctgccca tgaagccctg gaattccagc tccacctcca ctaccactcc aagcctggcc
     2581 ccaccagtgc tgtttggcct aggaactgtg gctgggaagg tgcctccaac aatgggatcc
     2641 agggaagcca aggagaagac agcccccctc ctatttcagc ctcctgcacc caaggcagtg
     2701 cctgagaagc ccatcataga caagaagtag taatgaacta gtccgtaact tgaaagtatt
     2761 tcgatttctt ggctttatat atcttgtgga aaggacgatt atgctgaaca ataatgtctg
     2821 cc
//
