LOCUS       sequence-28397-e         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP1
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    6..24
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    27..45
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     55..79
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      152..161
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             161..184
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             197..223
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    239..263
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agctctccct atcagtgata gagatctccc tatcagtgat agagatcgtc gacgagctcg
       61 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
      121 acaccgggac cgatccagcc tccggggatc tgccaccatg gactacaagg atgacgatga
      181 caagctcgat ggaggatacc catacgatgt tccagattac gctgctcgag gttatcaaac
      241 aagtttgtac aaaaaagttg gcatgcctgg tgtcatacct agtgaaagta atggactttc
      301 aagaggtagc ccttcaaaga aaaacagact ttccttaaag ttttttcaga aaaaggaaac
      361 taagagagct ttggatttca cagattctca agaaaatgaa gaaaaagctt ctgaatatag
      421 agcatctgaa attgatcaag ttgttcctgc agcacagtct tcacctataa actgtgagaa
      481 gagagaaaac ttgttaccat ttgtgggact gaataatctc ggcaatactt gctatcttaa
      541 tagtatactt caggtattat atttttgtcc cggttttaaa tctggagtaa agcacttatt
      601 taatattatt tcaaggaaga aagaagctct aaaggatgaa gccnatcaaa aagacaaggg
      661 aaattgcaaa gaagattctt tggcaagtta tgaattgata tgcagttt
//
