LOCUS       sequence-283971-        1238 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1299
FEATURES             Location/Qualifiers
     source          1..1238
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctcgaga atggagggcc gctcagggaa aggaccctgg gccctcagag
       61 aagcagaaac ctgttttcac accactgagg aggccaggca tgcttgtacc aaagcagaat
      121 gtttcacctc cagatatgtc agctttgtct ttaaaaggtg atcaagctct ccttggaggc
      181 attttttatt ttttgaacat ttttagccat ggcgagctac ctcctcattg tgaacagcgc
      241 ttcctccctt gtgaaattgg ctgtgttaag tattctctcc aagaaggtat tatggcagat
      301 ttccacagtt ttataaatcc tggtgaaatt ccacgaggat ttcgatttca ttgtcaggct
      361 gcaagtgatt ctagtcacaa gattcctatt tcaaattttg aacgtgggca taaccaagca
      421 actgtgttac aaaaccttta tagatttatt catcccaacc cagggaactg gccacctatc
      481 tactgcaagt ctgatgatag aaccagagtc aactggtgtt tgaagcatat ggcaaaggca
      541 tcagaaatca ggcaagatct acaacttctc actgtagagg accttgtagt ggggatctac
      601 caacaaaaat ttctcaagga gccctctaag acttggattc gaagcctcct agatgtggcc
      661 atgtgggatt attctagcaa cacaaggtgc aagtggcatg aagaaaatga tattctcttc
      721 tgtgctttag ctgtttgcaa gaagattgcg tactgcatca gtaattctct ggccactctc
      781 tttggaatcc agctcacaga ggctcatgta ccactacaag attatgaggc cagcaatagt
      841 gtgacaccca aaatggttgt attggatgca gggcgttacc agaagctaag ggttgggagt
      901 tcaggattct ctcatttcaa ctcttctaat gaggaacaaa gatcaaacac acccattggt
      961 gactacccat ctagggcaaa aatttctggc caaaacagca gcgttcgggg aagaggaatt
     1021 acccgcttac tagagagcat ttccaattct tccagcaata tccacaaatt ctccaactgt
     1081 gacacttcac tctcacctta catgtcccaa aaagatggat acaaatcttt ctcttcctta
     1141 tcttagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1201 tgtggaaagg acgaggtgga gcatcatgag aggtagct
//
