LOCUS       sequence-283980-        1457 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1336
FEATURES             Location/Qualifiers
     source          1..1457
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1324..1341
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 accgccacca tggccaccac cgcgcagtac ctgccgcggg gccccggtgg cggagccggg
       61 ggcaccgggc cgcttatgca cccggacgcc gcggcggcgg cggcggcggc ggcggccgcg
      121 gagcgattgc atgcaggggc cgcgtaccgc gaagtgcaga agctgatgca ccacgagtgg
      181 ctgggcgcgg gcgcgggcca ccccgtgggc ctagcgcacc cccagtggct acccacggga
      241 ggaggcggcg gcggcgattg ggccggcggc ccgcacctag aacacggcaa ggcaggcggc
      301 ggcggcaccg gccgagccga cgacggcggc ggcggcggag gtttccacgc gcgcctggtg
      361 caccaggggg cggcccacgc gggcgcggca tgggcgcagg gcagcacagc gcaccacttg
      421 ggcccggcca tgtcgccctc gcccggggcc agcgggggcc accagcccca gccgctcggg
      481 ctgtacgcgc aggcggccta cccggggggc ggcggcggcg gcctggccgg gatgctggcg
      541 gcgggcggtg gcggcgcggg gccgggcctg caccacgcgc tgcacgagga tggccacgag
      601 gcgcagctgg agccgtcgcc gccgccgcat ctgggcgccc acggacacgc acacggacat
      661 gcacacgcgg gcggcctgca cgcggcggcg gcgcacctgc acccgggcgc gggcggcggc
      721 ggctcatcgg tgggcgagca ctcggacgag gatgcgccca gctcggacga cctggagcag
      781 ttcgccaagc agttcaagca gcggcgcatc aagctgggct ttacgcaggc cgacgtgggg
      841 ctggcgctgg gcacgctcta cggtaacgtg ttctcgcaga ccaccatctg ccgcttcgag
      901 gccctgcagc tgagcttcaa gaacatgtgc aagctcaagc cgctgctcaa caagtggctg
      961 gaggagaccg actcgtccag cggcagcccc accaacctgg acaagatcgc ggcgcagggc
     1021 cgcaagcgca agaagcgcac gtccatcgag gtgggggtca aaggcgcgct cgagagccac
     1081 tttctcaagt gccccaagcc ctcggcgcac gagatcaccg gcttggcaga cagcctgcag
     1141 ctggagaagg aggtggtgcg cgtctggttc tgcaaccggc ggcagaagga gaagcgcatg
     1201 acccctgcgg ccggcgcggg ccacccgccc atggacgatg tatacgcgcc tggggagcta
     1261 gggcctggcg ggggcggcgc atcgccaccc tccgcgcccc caccgccccc gccggcggcg
     1321 ctgcaccacc accaccacca cacactgccc ggctcagtgc agtagtaatg aactagtccg
     1381 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgatccggtc
     1441 gaattgaaga acaccat
//
