LOCUS       sequence-283983-        4307 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1350
FEATURES             Location/Qualifiers
     source          1..4307
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggacac cgacctgttc atggaatgtg aggaggagga gttggagcca
       61 tggcagaaaa tcagtgatgt cattgaggac tctgtagttg aagattataa ttcagtggat
      121 aaaactacca cagtttctgt gagccagcag ccagtctcgg ctccagtgcc catcgctgcc
      181 catgcttctg ttgctgggca cctctctaca tccaccaccg ttagtagcag cggggcacag
      241 aacagcgaca gtacaaagaa gactcttgtc acactaattg ccaacaacaa tgctggcaat
      301 cctttggtcc agcaaggtgg acagccactc atcctgaccc agaatccagc cccaggtctg
      361 ggcacaatgg ttactcaacc agtattgagg cctgttcagg tcatgcagaa tgccaatcat
      421 gtgactagtt cccctgtggc ctcacaacca atatttatca ctacgcaggg atttcctgta
      481 aggaatgtcc ggcctgtaca aaatgcaatg aatcaggttg ggattgtgct gaacgtacag
      541 caaggccaaa cggttagacc aattacacta gttccagccc caggtaccca gtttgttaag
      601 ccgacagttg gagttccaca agtgttctcc cagatgaccc ctgtgaggcc aggctccaca
      661 atgcctgtga ggcccaccac caacaccttc accaccgtca tcccggccac tcttaccatt
      721 cgaagcaccg tcccacagtc ccagtcccag cagaccaagt ccactcccag cacttctacc
      781 actcccactg ccacacagcc aacctcactg gggcaactag ctgttcagtc tccaggccag
      841 tcaaaccaga ccacgaatcc caagctagtg agcattgcca gctttgtcac tgtgaagcga
      901 cctggtgtta caggcgaaaa tagcaatgaa gtggccaaat tggtgaatac ccttaacacc
      961 atcccttccc tgggccagag tcctgggcca gtggtggtgt ccaacaacag ctctgctcat
     1021 ggctctcaaa gaaccagcgg acctgagtct tcaatgaaag tgacctcttc catcccagta
     1081 tttgacctcc aggatggtgg acggaaaata tgtccacgat gtaatgctca atttcgtgtt
     1141 actgaagctt tgagaggtca catgtgttac tgttgcccag aaatggttga ataccagaag
     1201 aaaggaaagt ccctggattc agaacccagt gtcccatcag cagcaaagcc cccatcccct
     1261 gagaaaacag ctcctgttgc ttccacaccc tcttctacac ctattcctgc tctgtcaccg
     1321 cctaccaaag taccagaacc aaatgagaac gtgggcgatg ccgtccagac caaactcatt
     1381 atgcttgtag atgacttcta ctatggacgg gatggtggca aagtagccca gctcacaaat
     1441 ttccctaagg tcgccacatc tttccgatgc ccacattgta ccaaaaggct aaaaaacaat
     1501 attcgattca tgaaccatat gaaacaccac gtagaactcg atcagcagaa cggtgaggta
     1561 gatggtcaca ctatctgcca gcactgttac cgccagtttt ccactccctt ccagcttcag
     1621 tgccacttgg aaaatgttca tagtccctat gaatctacta ccaagtgcaa gatctgtgaa
     1681 tgggcgtttg aaagtgagcc actatttctc cagcatatga aggatactca taagcctgga
     1741 gagatgcctt atgtttgcca ggtgtgtcaa tatcgctcct cactctactc tgaggtagat
     1801 gtccattttc ggatgatcca tgaggatacc cggcatctgc tctgccctta ttgcctgaag
     1861 gtcttcaaaa atggcaatgc attccaacag cattacatga ggcaccagaa gagaaatgtt
     1921 tatcactgca acaaatgccg gctgcagttt ctctttgcca aggacaaaat tgaacacaag
     1981 cttcaacacc ataaaacctt ccgtaaaccc aagcagctgg agggcttgaa accaggcacc
     2041 aaggtgacaa tccgggcttc ccgagggcag ccacgaactg ttcctgtatc ctctaatgat
     2101 acacctccca gcgccttgca ggaggcagca ccgctgacct cctcaatgga ccctctgcct
     2161 gtcttccttt atccccctgt ccagcgcagc atccagaaga gagctgttag gaaaatgagt
     2221 gtcatgggcc ggcagacatg cctggagtgc agcttcgaga tcccagactt ccctaatcat
     2281 ttccctactt acgtacactg ctctctgtgt cgctatagca cctgctgttc tcgagcttat
     2341 gccaaccaca tgatcaacaa tcatgttcca cggaagagcc ccaagtattt ggctttgttt
     2401 aaaaattctg tgagtggaat caagctggcc tgcacttcat gtacctttgt tacctctgtg
     2461 ggcgatgcta tggccaagca tttggtattc aacccctctc acagatccag cagcatcctg
     2521 ccacggggac tcacttggat agctcactca aggcatggcc agactcgtga ccgagtgcat
     2581 gaccggaacg tgaagaatat gtaccctcct ccttccttcc ccactaacaa agctgccact
     2641 gtgaaatctg cgggggccac cccagctgag cctgaagagc tactaactcc cttagcccca
     2701 gcactcccat caccagcctc aactgcaacc ccaccaccaa cccccactca cccgcaggct
     2761 ttagcccttc caccgctggc tacagaggga gccgaatgtc tgaatgttga tgatcaggat
     2821 gaagggagcc cagtcaccca agaacctgag ctagcatcag gtggtggtgg tagtggtgga
     2881 gttggcaaaa aggagcagct gtctgtgaag aagcttcgag tagtactgtt tgctctatgc
     2941 tgcaatacag aacaggcagc tgaacacttc cgaaatcccc agcgacgtat tcgccgttgg
     3001 cttcgacgtt tccaggcctc ccagggggag aatctagagg gcaaatatct gagctttgag
     3061 gcagaagaga aactggctga gtgggtgcta acccagcgcg aacaacagct acctgtaaat
     3121 gaggagacct tgttccagaa ggccaccaaa ataggacgtt ctttggaagg ggggtttaag
     3181 atctcctatg agtgggctgt gcgtttcatg ctgcggcacc acctgactcc ccatgcccgg
     3241 cgagctgtgg cccacaccct acctaaggat gtagcagaga atgcaggact cttcattgat
     3301 tttgtacaac ggcagattca caaccaggac ttacccttgt ctatgattgt ggctattgat
     3361 gagatctctt tgttcctgga tacagaggtg ctgagcagtg atgatcgaaa ggagaatgcc
     3421 ctgcagacag tgggcacagg ggaaccttgg tgtgatgtag tcctagccat tctggcagat
     3481 ggcactgtcc ttcccaccct ggttttctac agagggcaga tggatcagcc tgctaacatg
     3541 ccagactcca tattgctaga ggcaaaggag agtggctaca gtgatgacga gatcatggag
     3601 ctgtggtcaa ctcgagtgtg gcagaagcac acagcttgcc agcgcagcaa aggcatgctt
     3661 gtgatggact gtcatcgcac tcacttgtca gaagaggtac tggctatgct tagtgcctct
     3721 agcactttgc ctgcagtggt cccagcaggc tgtagctcca aaattcagcc attagatgta
     3781 tgcatcaaaa gaactgtcaa gaacttcctg cataaaaaat ggaaggaaca ggctcgggaa
     3841 atggcagata ctgcatgtga ttctgatgtc ctgcttcagc tggtgcttgt ctggctgggt
     3901 gaagtgctag gtgtcattgg ggactgtcca gagctagttc agcgctcctt cctggtggct
     3961 agtgttctgc ctggccccga tggcaacatt aactcaccta caagaaatgc tgacatgcag
     4021 gaggagctaa ttgcctccct agaggagcaa ctgaagctga gtggggaaca ttctgagtct
     4081 tccactccac gacccagatc atctcctgaa gagacaattg agcctgaaag tcttcaccag
     4141 ctctttgagg gtgaaagtga gaccgagtct ttctatggct ttgaagaagc tgacctagat
     4201 ctgatggaga tttagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     4261 tatatatctt gtggaaagga cgactgggag ctactgcaaa gcctatt
//
