LOCUS       sequence-283995-        1742 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1378
FEATURES             Location/Qualifiers
     source          1..1742
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccgaagc ggcgcctgcc cgggaccccg agacagacaa gcacacagag
       61 gaccagagtc cttcgacacc cttgccccag ccagctgctg agaagaactc gtacctctac
      121 tccacggaaa tcacactgtg gacggtggtg gccgccattc aggccttgga gaagaaggtg
      181 gattcctgcc tgacccgctt gctgactctg gaggggcgca cggggacagc cgagaagaag
      241 ctggccgact gcgagaagac agctgtggag ttcgggaacc agctggaggg caagtgggcc
      301 gtgctgggga ccttgctgca ggagtacggg ctgctgcaga ggcggctgga gaatgtggag
      361 aacttgctgc gcaacaggaa cttctggatc ttgcggctgc ccccgggcag caagggggag
      421 gcccccaagg tgcccgtgac cttcgatgat gtggccgtgt atttctctga gctggagtgg
      481 ggcaagctgg aggactggca gaaggagctc tacaagcacg tgatgagggg caactacgag
      541 acgctggtct ccctggatta tgcaatctcc aaaccagaca tcctcacccg gatagagagg
      601 ggagaggagc cttgtcttga ccggtggggc caggagaagg ggaatgaagt agaggtggga
      661 cgtccaagga tgatgggcac tggcctccct ccgtatccag agcacctcac cagcccactt
      721 agccctgccc aggaggagct gaaagaaggg caggccccca agcagcagca ggactcagag
      781 gcgagagtgg ccccagccgg gccagaagca ggtggtggtg tggccatcaa gacagaggca
      841 cagtctgaag acgagatgac gcctgagcgg ctctttctgg gggtgtcccg aggccagacc
      901 gagtgtagaa tcccccgagg gcccaggaac aggcctgggg gccccagccg tcatcaggcc
      961 cagggcatgc ccagggtgcg ggcaggggag ccacggccac cgggggccag tggggagacg
     1021 ccccgagtcc tctcccgcag gcggcagcgg gcattcccct gccccgactg cgggcagagc
     1081 ttccgcctga agatcaatct gacgattcat cagcggaccc atgtggagga ggggcggcag
     1141 gaggcccccg gccgctcgcc caccagctgc ggggacagcc aggccatgct ggagccgggg
     1201 gaggtggtgg tacccggccc tgtcatccgc tggctccccg aggagcctga gggtcgccgc
     1261 tccgtggcag ggggccgtgc cttggtgggg cggcggcctg cagccagcaa gatgtaccac
     1321 tgcagcgagt gcctgcgctt cttccagcag cgcaagagcc tgctgctgca ccagcgcctg
     1381 cacaccggca atggccaggg ctggcccgcc tgcccctact gcggcaaggc cttccgccgg
     1441 ccctcggacc tcttccggca ccagcgcatc cacaccggtg agcggcccta ccagtgcccc
     1501 cagtgtggcc ggaccttcaa ccgcaaccac cacctggccg tgcacatgca gacccacgcc
     1561 cgaggccagg tgggcccaca cttccctgcc gcccccgccc gccacgggag cctgcccctg
     1621 ccctggccca gccggaagga ggagggctag taatgaacta gtccgtaact tgaaagtatt
     1681 tcgatttctt ggctttatat atcttgtgga aaggacgagg tgatcttgga ggctcggttt
     1741 ga
//
