LOCUS       sequence-28401-e         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP33
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    2..20
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     30..54
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      127..136
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             136..159
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             172..198
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    214..238
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctccctatca gtgatagaga tcgtcgacga gctcgtttag tgaaccgtca gatcgcctgg
       61 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      121 ggatctgcca ccatggacta caaggatgac gatgacaagc tcgatggagg atacccatac
      181 gatgttccag attacgctgc tcgaggttat caaacaagtt tgtacaaaaa agttggcatg
      241 acaggatcaa attcacacat aacgatatta accttaaagg tgttacctca ttttgaaagt
      301 cttgggaaac aggaaaaaat tcctaacaaa atgtcagctt ttcgaaatca ttgtccacat
      361 ttggattcag ttggtgaaat aacaaaagaa gatttgatac aaaaatccct tggtacttgt
      421 caggattgta aagtccaagg accaaatctt tgggcatgtc tggagaatag atgttcatat
      481 gttggctgtg gtgaatcaca agtagatcac agcaccatac attctcagga gacaaagcat
      541 tatctaactg tgaaccttac cactcttcga gtatggtgtt atgcttgcag caaagaagta
      601 tttttggata ggaaattagg aactcagcct tcattgcctc atgtaagaca acctcaccaa
      661 atacaagaaa acagtgtcca ggantttaaa atacccagta atacaacatt aaaaactcct
      721 ctggttgccg tatttgatga tctggatata gaagcggatg aagaaga
//
