LOCUS       sequence-284010-        3821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1531
FEATURES             Location/Qualifiers
     source          1..3821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagctccc caactacagc cggcagctgc tgcagcagct gtacactctg
       61 tgcaaggagc agcagttctg tgattgcacc atctccattg gtaccattta cttcagggct
      121 cacaagcttg tcctggctgc tgccagcctc ctgttcaaaa ccctgctgga taacacagat
      181 accatctcca tcgatgcatc tgtggtgagc cccgaggagt ttgcgctctt gttggaaatg
      241 atgtacacgg gcaaactacc tgtgggcaag cacaacttct ccaaaatcat ctccttagca
      301 gacagtctac agatgtttga tgtagctgtt agctgcaaaa atcttctgac cagccttgta
      361 aactgctcgg ttcagggtca ggtggtaagg gatgtctctg cgccatcctc agagacattc
      421 agaaaggaac cagagaagcc tcaagtagaa atcctttcat ctgaaggtgc tggagagcct
      481 cattcttccc cagagcttgc tgccactcca gggggccctg tgaaagctga gactgaggaa
      541 gcagcccatt cagtttcaca agagatgagt gtgaattctc ccacagccca ggagagccag
      601 aggaatgcag aaaccccagc ggagactcct actacagctg aagcttgttc cccctcccct
      661 gctgtgcaaa cctttagtga ggcaaagaag acaagcacag aaccaggatg tgaaaggaaa
      721 cactaccagc tgaattttct tctagaaaat gaaggtgtct tctcagatgc actcatggtt
      781 acccaggatg ttttaaaaaa actagaaatg tgttcagaaa ttaaaggtcc acagaaggag
      841 atgatagtga aatgtttcga gggtgaagga ggacattcag cattccagag aatcctgggt
      901 aaagtaagag aggaaagcct ggatgttcaa actgttgtgt ccctgttgag gctgtaccaa
      961 tattctaatc ctgcagtaaa aacagcacta ttagacagga agccagaaga tgtagacaca
     1021 gtgcagccaa aagggagcac agaggaggga aagaccttgt ctgttctgtt actagaacac
     1081 aaagaggacc tgatacagtg tgtaacacag ctgagaccta ttatggagtc cctggaaaca
     1141 gccaaggagg aattcctgac tggcactgaa aaaagggtga ttctgaattg ctgtgagggc
     1201 agaacaccca aggagacaat agaaaatttg ttgcacagaa tgactgaaga gaagacgctg
     1261 actgctgagg gtttggtaaa actcctccag gctgtgaaga cgactttccc aaacctgggc
     1321 cttctgctag agaagttgca gaaatcagcc actttgccaa gcaccacagt ccaaccaagc
     1381 cctgatgatt atgggactga gctattgaga cgctatcatg aaaacctctc tgagattttc
     1441 acagacaacc agattttatt aaagatgatc tcacacatga caagtttagc ccctggagaa
     1501 agagaggtca tggagaagct tgtgaaacgt gactctggtt caggtggttt caattctctg
     1561 atatcagcag ttctagaaaa gcagactctc tctgccacag ccatttggca actgctgctg
     1621 gtggttcagg agacaaagac ctgtccattg gacctgctca tggaggaaat acgaagggag
     1681 cctggtgccg atgctttctt ccgggcagtg accaccccag aacatgccac tttagaaaca
     1741 atcctgaggc ataaccagtt gatcttggag gccatccaac agaagattga gtacaagctc
     1801 tttacctcgg aggaggagca cctggcagag actgtgaaag agattctgag cattccctct
     1861 gagacagcca gccctgaagc ttccctgaga gcagtgctga gcagagccat ggaaaaatca
     1921 gtcccggcca ttgaaatatg ccacctcctg tgcagtgtcc acaaatcttt tccaggcctg
     1981 cagcctgtca tgcaggagtt ggcatacatt ggtgtcctta ctaaggaaga tggagagaag
     2041 gaaacgtgga aggtgagtaa taaatttcac cttgaagcca acaacaaaga agatgaaaag
     2101 gcagccaaag aagacagcca gcctggggaa cagaatgatc aaggagagac tggttccttg
     2161 ccaggacagc aagagaaaga ggcttcagcc tccccagacc ctgccaagaa gagcttcatc
     2221 tgtaaggcct gcgacaaaag cttccatttc tactgccgcc taaaggtgca catgaagcgc
     2281 tgccgggtgg ctaagagcaa acaggtgcag tgtaaggagt gcagtgagac caaggattca
     2341 aagaaagagc tggacaaaca tcagctggag gcccatggtg caggtggaga gcccgatgcc
     2401 cccaagaaga agaagaagag gcttccagtg acatgtgacc tctgtggcag agaatttgcc
     2461 catgcctcag gcatgcagta ccataagctg acagagcact tcgatgagaa gcctttctcc
     2521 tgtgaagagt gtggggcgaa gtttgcagcc aattccaccc tgaagaacca ccttcgcctt
     2581 cacaccgggg accgcccgtt catgtgcaag cactgcctca tgaccttcac ccaggcctcc
     2641 gccctggcct atcacaccaa gaagaagcac tcagaaggga aaatgtatgc atgccagtac
     2701 tgtgatgctg tgtttgccca gtctattgag ctgtcccgcc acgtgaggac ccacaccggg
     2761 gacaagccct atgtctgcag agactgtggc aagggcttcc ggcaagccaa tggcctctcc
     2821 atccatctgc acacctttca caacatagaa gatccttatg actgcaagaa gtgcaggatg
     2881 agtttcccca ctcttcagga tcaccggaag cacatccatg aggtgcactc caaagagtac
     2941 cacccctgcc ccacgtgtgg gaagatcttc agtgccccgt ccatgctgga gcggcacgtg
     3001 gtgacccacg ttggagggaa gcccttcagc tgcgggatct gcaacaaggc ctaccagcaa
     3061 ttgtctggtt tgtggtacca caatcgaacc caccaccctg acgtatttgc tgctcagaac
     3121 caccgatctt ccaagttctc atcactccag tgcagctcct gtgacaaaac cttccccaac
     3181 accattgagc acaagaagca catcaaagca gaacatgcag atatgaagtt ccatgaatgt
     3241 gaccagtgta aggagctctt ccccacgcca gccttgctgc aggttcatgt caagtgccag
     3301 cattcagggt cccagccatt ccggtgcttg tactgtgctg ctactttccg ttttcctgga
     3361 gcattgcagc accatgtcac cacggagcac ttcaagcagt cagagaccac cttcccctgt
     3421 gagctctgtg gggaactctt cacctcccag gcccagcttg acagtcacct ggaatctgag
     3481 cacccaaagg tgatgagcac ggaaacccag gccgcagcct cacagatggc gcaggtgatc
     3541 caaaccccag agccggtggc cccgacagag caggtgatca ctttggagga gacccagctt
     3601 gccgggtcgc aggtgtttgt gacgttgcca gattctcagg catctcaggc cagctctgag
     3661 ctcgtggcgg tgactgtgga ggacttgctg gatggcacag tgacgctgat ctgtggtgag
     3721 gccaaatagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     3781 tcttgtggaa aggacgaatt tcaaactgct ctgctgatat c
//
