LOCUS       sequence-284025-        1697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1686
FEATURES             Location/Qualifiers
     source          1..1697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtgtccaa gctcacgtcg ctccagcaag aactcctgag cgccctgctg
       61 agctccgggg tcaccaagga ggtgctggtt caggccttgg aggagttgct gccatccccg
      121 aacttcgggg tgaagctgga gacgctgccc ctgtcccctg gcagcggggc cgagcccgac
      181 accaagccgg tcttccatac tctcaccaac ggccacgcca agggccgctt gtccggcgac
      241 gagggctccg aggacggcga cgactatgac acacctccca tcctcaagga gctgcaggcg
      301 ctcaacaccg aggaggcggc ggagcagcgg gcggaggtgg accggatgct cagtgaggac
      361 ccttggaggg ctgctaaaat gatcaagggt tacatgcagc aacacaacat cccccagagg
      421 gaggtggtcg atgtcaccgg cctgaaccag tcgcacctct cccagcatct caacaagggc
      481 acccctatga agacccagaa gcgtgccgct ctgtacacct ggtacgtcag aaagcaacga
      541 gagatcctcc gacagttcag tcaacagagc catgggcctg ggcagtccga tgatgcctgc
      601 tctgagccca ccaacaagaa gatgcgccgc aaccggttca aatgggggcc cgcgtcccag
      661 caaatcttgt accaggccta cgatcggcaa aagaacccca gcaaggaaga gagagaggcc
      721 ttagtggagg aatgcaacag ggcagaatgt ttgcagcgag gggtgtcccc ctccaaagcc
      781 cacggcctgg gctccaactt ggtcactgag gtccgtgtct acaactggtt tgcaaaccgc
      841 aggaaggagg aggcattccg gcaaaagctg gccatggacg cctatagctc caaccagact
      901 cacagcctga accctctgct ctcccacggc tccccccacc accagcccag ctcctctcct
      961 ccaaacaagc tgtcaggagt gcgctacagc cagcagggaa acaatgagat cacttcctcc
     1021 tcaacaatca gtcaccatgg caacagcgcc atggtgacca gccagtcggt tttacagcaa
     1081 gtctccccag ccagcctgga cccaggccac aatctcctct cacctgatgg taaaatgatc
     1141 tcagtctcag gaggaggttt gcccccagtc agcaccttga cgaatatcca cagcctctcc
     1201 caccataatc cccagcaatc tcaaaacctc atcatgacac ccctctctgg agtcatggca
     1261 attgcacaaa gcctcaacac ctcccaagca cagagtgtcc ctgtcatcaa cagtgtggcc
     1321 ggcagcctgg cagccctgca gcccgtccag ttctcccagc agctgcacag ccctcaccag
     1381 cagcccctca tgcagcagag cccaggcagc cacatggccc agcagccctt catggcagct
     1441 gtgactcagc tgcagaactc acacatgtac gcacacaagc aggaaccccc ccagtattcc
     1501 cacacctccc ggtttccatc tgcaatggtg gtcacagata ccagcagcat cagtacactc
     1561 accaacatgt cttcaagtaa acagtgtcct ctacaagcct ggtagtaatg aactagtccg
     1621 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgagccactt
     1681 cgcaactcgc cgctctg
//
