LOCUS       sequence-28403-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP42
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..25
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    28..46
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     56..80
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      153..162
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             162..185
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             198..224
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    240..264
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gagctctccc tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc
       61 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
      121 gacaccggga ccgatccagc ctccggggat ctgccaccat ggactacaag gatgacgatg
      181 acaagctcga tggaggatac ccatacgatg ttccagatta cgctgctcga ggttatcaaa
      241 caagtttgta caaaaaagtt ggcatgacca tagttgacaa agcttctgaa tcttcagacc
      301 catcagccta tcagaatcag cctggcagct ccgaggcagt ctcacctgga gacatggatg
      361 caggttctgc cagctggggt gctgtgtctt cattgaatga tgtgtcaaat cacacacttt
      421 ctttaggacc agtacctggt gctgtagttt attcgagttc atctgtacct gataaatcaa
      481 aaccatcacc acaaaaggat caagccctag gtgatggcat cgctcctcca cagaaagttc
      541 ttttcccatc tgagaagatt tgtcttaagt ggcaacaaac tcatagagtt ggagctgggc
      601 tccagaattt gggcaatacc tgttttgcca atgcagcact gcagtgttta acctacacac
      661 cacctcttgc caattacatg ctatcacatg aacactccaa aacatgtcat gcagaaggct
      721 tttgtatgat gtgtacaatg caagcacata ttacccaggc actcagtaat cctggggacg
      781 ttattaaacc aatgtttgtc atcaatgaga tgcggcgtat agct
//
