LOCUS       sequence-284043-        2342 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1746
FEATURES             Location/Qualifiers
     source          1..2342
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctttttc tcagggtcta ttgacattca gggatgtggc catagaattc
       61 tctcaggagg agtggaaatg cctggaccct gctcagagga ctctatacag ggacgtgatg
      121 ctggagaatt ataggaacct ggtctccctg gatatctctt ccaaatgcac gatgaaggag
      181 ttcttgtcaa cagcgcaagg caacagagaa gtgttccatg cagggacatt gcaaatacat
      241 gaaagtcatc acaatggaga tttttgctac caggatgttg ataaagatat tcatgactat
      301 gaatttcaat ggcaagaaga tgaaagaaat ggccatgaag cacccatgac aaaaatcaaa
      361 aagttgacag gtattacaga acgatatgat caaagtcatg ctagaaacaa gcctattaaa
      421 gatcagcttg gatcaagctt tcattcgcat ctgcctgaaa tgcacatatt tcagaccgaa
      481 gagaaaattg ataatcaagt tgtgaagtct gtccacgatg cttccttggt ttcaacagcc
      541 caaagaattt cttgtaggcc caaaacccat atatctaata accatgggaa taatttctgg
      601 aattcttcat tactcacaca aaaacaggaa gtacacatga gagaaaaatc tttccaatgt
      661 aatgagagtg gcaaagcctt taattacagc tcactcttaa ggaaacatca gataatccat
      721 ttagcagaca aatataaatg tgatgtatgt ggcaagctct ttaatcagaa gcgaaaccta
      781 gcatgccatc gtagatgtca cactggtgag aatccttaca agtgtaatga gtgtggcaag
      841 accttcagtc agacgtcatc ccttacatgc catcgtagac ttcatactgg agagaaacct
      901 tacaaatgtg aagaatgtga caaagctttc catttcaaat caatacttga aagacatagg
      961 ataattcata ctgaagagaa accatataag tgtaatgagt gtggcaagac ctttaggcag
     1021 aagtcaatcc ttacacgcca tcatcgactt catactggag agaaacctta caagtgtaat
     1081 gagtgtggca agacctttag tcacaagtca tcccttacat gccatcatag acttcatact
     1141 ggagagaaac cctacaaatg taatgagtgt ggcaagacct ttagtcacaa gtcatccctt
     1201 acatgccatc gtagacttca tactggagag aaaccttaca aatgtgaaga atgtgacaaa
     1261 gcttacagtt tcagatcaaa ttttgaaata catcggaaaa ttcatactga agacaatgct
     1321 tacaagtgta atgagtgtgg aaagaccttt agccggacat catcccttac atgccatcgt
     1381 agacgtcata ctggagagca accttacaaa tgtgaagaat gtgacaaagc tttccgtttc
     1441 aaatcaaacc ttgaaagaca taggagaatt catactggag agaaaccata caagtgtaat
     1501 gagtgtggca agacctttag tcggaagtca tacctcacat gccatcatag acttcatact
     1561 ggagagaaag cttacaagtg taatgagtgc ggcaagacct ttagttggaa gtcatccctt
     1621 acctgccatc gtagacttca ttctggagag aaaccttaca agtgtaagga gtgtggcaag
     1681 accttcaatc agcagttaac ccttaaacgc catcgtagac ttcatagtgg agagaaccct
     1741 tacaaatgtg aagatagtga caaagcttac agtttcaaat caaaccttga aatacatcag
     1801 aaaattcata ctgaagagaa tccttacaag tgtaatgagt gtggcaagac cttcagtcgg
     1861 acgtcatccc ttacatgcca tcgtagactt cataccggag agaaacctta caaatgtgaa
     1921 gaatgtgaca aagctttccg tgtgaaatca aaccttgaag gacataggag aattcatact
     1981 ggagagaaac cttacaagtg taatgagtgt ggcaagacct ttagtcggaa gtcatatttt
     2041 atatgccatc atagacttca tactggagag aaaccttata agtgtaatga gtgtggcaag
     2101 aactttagtc agaagtcatc ccttatatgc caccatagac ttcatactgg agagaaacct
     2161 tacaagtgta atgagtgtgg caagaccttt agtcagaagt caaaccttac atgccatcgt
     2221 agacttcata ctggagaaaa acaagtgtag taatgaacta gtccgtaact tgaaagtatt
     2281 tcgatttctt ggctttatat atcttgtgga aaggacgacg gcgcgggctc gagtgtgaag
     2341 ga
//
