LOCUS       sequence-284046-        2357 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1749
FEATURES             Location/Qualifiers
     source          1..2357
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaagatct gtcctctcca gactccaccc ttctccaagg gggacataat
       61 ctactctcat cagccagttt tcaggaagcg gtgactttca aggatgtgat agtggacttt
      121 acccaggaag aatggaaaca gctggaccct ggccagagag atttgttcag ggatgtgaca
      181 ttggaaaatt atacacacct ggtctctata ggactccaag tttctaaacc tgatgtgatt
      241 tcccagttag agcaagggac agagccatgg atcatggagc caagcattcc agtaggtacc
      301 tgtgcagact gggagacaag acttgaaaat agtgtgtcag ccccagagcc tgacatttct
      361 gaagaagagc tatctccaga ggtaatagtg gaaaaacaca aaagagatga ttcttggagt
      421 tccaacttgc tagaaagttg ggaatatgaa ggcagtttag agagacagca ggcaaaccaa
      481 cagacactgc caaaggaaat aaaggtaacc gaaaagacaa tacccagttg ggaaaaaggc
      541 cctgtaaata atgaatttgg gaaaagtgtc aatgtgagtt caaaccttgt aacacaagaa
      601 ccatctccag aagagacctc tactaaaaga agcatcaaac agaattcaaa cccagttaaa
      661 aaagagaaat cttgtaagtg caatgaatgt gggaaagcct ttagttattg ttcagctctt
      721 attcgccatc agagaacaca tactggagaa aaaccctaca aatgtaatga atgtgaaaaa
      781 gccttcagcc ggagtgaaaa ccttataaac catcaaagaa ttcatactgg agataaacca
      841 tataaatgtg atcagtgtgg aaaaggcttc attgagggtc catctcttac tcaacatcaa
      901 agaattcata ctggagaaaa accatataaa tgtgatgaat gtgggaaagc ctttagtcag
      961 aggacccatc ttgttcagca tcagagaatt catactggcg agaagccata cacttgtaat
     1021 gagtgtggaa aagcctttag ccagagaggc cactttatgg aacatcagaa aattcatacg
     1081 ggagaaaaac cttttaaatg tgatgaatgt gataaaacct tcaccaggag cacacacctt
     1141 actcaacatc aaaaaattca tactggagaa aaaacctata aatgtaatga atgtggaaag
     1201 gccttcaacg ggccctcaac ttttatccgt catcatatga ttcatactgg tgaaaaaccg
     1261 tacgaatgca atgaatgtgg gaaagccttc agccagcact caaacctcac tcagcatcaa
     1321 aaaactcata ctggggagaa accctatgat tgtgcagaat gtggaaaatc ttttagttac
     1381 tggtcatccc ttgctcaaca cctgaaaatt catactggag aaaaacctta caaatgcaat
     1441 gaatgtggaa aggccttcag ttactgctca tcccttactc aacatcggag aattcacacg
     1501 agagaaaagc cctttgaatg cagtgaatgt ggaaaggctt tcagttatct ctcaaacctt
     1561 aatcagcatc agaaaactca tactcaagag aaagcttatg aatgtaaaga atgtgggaaa
     1621 gcttttattc ggagttcatc tcttgctaag catgaaagaa ttcatactgg agagaaaccc
     1681 tatcagtgtc atgaatgtgg gaaaaccttc agttatggtt catcccttat tcagcatagg
     1741 aagatccata ctggagaacg accttacaag tgtaatgagt gtgggagagc attcaaccag
     1801 aacatacacc ttacacagca taagagaatt catacaggag ccaagcctta tgagtgtgct
     1861 gagtgtggta aagcctttcg acattgttca tctcttgctc aacatcaaaa aactcacaca
     1921 gaagaaaaac cctaccagtg taataaatgt gaaaagacct ttagccagag ctcccatcta
     1981 actcagcatc aacgaattca cactggggag aagccctata agtgcaatga atgtgacaaa
     2041 gcctttagcc ggagcactca tctgactgaa catcagaata ctcatactgg agagaaacct
     2101 tataactgta atgaatgcag aaagactttt agccagagca catatctcat tcagcaccag
     2161 agaattcatt caggagagaa gccttttgga tgtaatgatt gtggaaaatc cttcagatat
     2221 cgctctgctc tcaacaaaca tcagagactg catcctggca tatagtaatg aactagtccg
     2281 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgattctgca
     2341 gaggttggtc gccatcg
//
