LOCUS       sequence-284067-        2489 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1816
FEATURES             Location/Qualifiers
     source          1..2489
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatcattt gaacgaggca actcagggga aagaacattc agaaatgtct
       61 aacaatgtga gtgatccgaa gggtccacca gccaagattg cccgcctgga gcagaacggg
      121 agcccgctag gaagaggaag gcttgggagt acaggtgcaa aaatgcaggg agtgccttta
      181 aaacactcgg gccatctgat gaaaaccaac cttaggaaag gaaccatgct gccagttttc
      241 tgtgtggtgg aacattatga aaacgccatt gaatatgatt gcaaggagga gcatgcagaa
      301 tttgtgctgg tgagaaagga tatgcttttc aaccagctga tcgaaatggc attgctgtct
      361 ctaggttatt cacatagctc tgctgcccag gccaaagggc taatccaggt tggaaagtgg
      421 aatccagttc cactgtctta cgtgacagat gcccctgatg ctacagtagc agatatgctt
      481 caagatgtgt atcatgtggt cacattgaaa attcagttac acagttgccc caaactagaa
      541 gacttgcctc ccgaacaatg gtcgcacacc acagtgagga atgctctgaa ggacttactg
      601 aaagatatga atcagagttc attggccaag gagtgccccc tttcacagag tatgatttct
      661 tccattgtga acagtactta ctatgcaaat gtctcagcag caaaatgtca agaatttgga
      721 aggtggtaca aacatttcaa gaagacaaaa gatatgatgg ttgaaatgga tagtctttct
      781 gagctatccc agcaaggcgc caatcatgtc aattttggcc agcaaccagt tccagggaac
      841 acagccgagc agcctccatc ccctgcgcag ctctcccatg gcagccagcc ctctgtccgg
      901 acacctcttc caaacctgca ccctgggctc gtatcaacac ctatcagtcc tcaattggtc
      961 aaccagcagc tggtgatggc tcagctgctg aaccagcagt atgcagtgaa tagactttta
     1021 gcccagcagt ccttaaacca acaatacttg aaccaccctc cccctgtcag tagatctatg
     1081 aataagcctt tggagcaaca ggtttcgacc aacacagagg tgtcttccga aatctaccag
     1141 tgggtacgcg atgaactgaa acgagcagga atctcccagg cggtatttgc acgtgtggct
     1201 tttaacagaa ctcagggctt gctttcagaa atcctccgaa aggaagagga ccccaagact
     1261 gcatcccagt ctttgctggt aaaccttcgg gctatgcaga atttcttgca gttaccggaa
     1321 gctgaaagag accgaatata ccaggacgaa agggaaagga gcttgaatgc tgcctcggcc
     1381 atgggtcctg cccccctcat cagcacacca cccagccgtc ctccccaggt gaaaacagct
     1441 actattgcca ctgaaaggaa tgggaaacca gagaacaata ccatgaacat taatgcttcc
     1501 atttatgatg agattcagca ggaaatgaag cgtgctaaag tgtctcaagc actgtttgca
     1561 aaggttgcag caaccaaaag ccagggatgg ttgtgcgagc tgttacgctg gaaagaagat
     1621 ccttctccag aaaacagaac cctgtgggag aacctctcca tgatccgaag gttcctcagt
     1681 cttcctcagc cagaacgtga tgccatttat gaacaggaga gcaacgcggt gcatcaccat
     1741 ggcgacaggc cgccccacat tatccatgtt ccagcagagc agattcagag cccctctccc
     1801 accacccttg ggaaaggaga gtctagaggc gttttcttac caggcctgcc gacccctgca
     1861 ccatggctcg gtgctgctcc tcagcaacag cagcagcaac agcaacagca gcagcagcag
     1921 cagcaggcac cgccgcctcc acagccacag cagcagccac agacaggccc tcggctcccc
     1981 ccacggcaac ccacggtggc ctctccagca gagtcagatg aggaaaaccg acagaagacc
     2041 cggccacgaa caaaaatttc agtggaagcc ttgggaatcc tccagagttt catacaagac
     2101 gtgggcctgt accctgacga agaggccatc cagactctgt ctgcccagct cgaccttccc
     2161 aagtacacca tcatcaagtt ctttcagaac cagcggtact atctcaagca ccacggcaaa
     2221 ctgaaggaca attccggttt agaggtcgat gtggcagaat ataaagaaga ggagctgctg
     2281 aaggatttgg aagagagtgt ccaagataaa aatactaaca cccttttttc agtgaaacta
     2341 gaagaagagc tgtcagtgga aggaaacaca gacattaata ctgatttgaa agactagtaa
     2401 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     2461 gacgatcagg gtgggtaggc agtcctccg
//
