LOCUS       sequence-28407-e         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP53
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..31
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     41..65
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      138..147
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             147..170
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             183..209
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    225..249
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtgatagaga tctccctatc agtgatagag atcgtcgacg agctcgttta gtgaaccgtc
       61 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      121 ccagcctccg gggatctgcc accatggact acaaggatga cgatgacaag ctcgatggag
      181 gatacccata cgatgttcca gattacgctg ctcgaggtta tcaaacaagt ttgtacaaaa
      241 aagttggcat ggtttgtcag ggagatgcct gtatattttg tgcattgaag acgatatttg
      301 cacagttcca acacagtcga gaaaaagcac ttccctcaga taacataagg catgctcttg
      361 cagaaagttt caaagatgag cagcgatttc aacttggcct tatggatgat gctgcggagt
      421 gctttgaaaa tatgttggag aggattcatt ttcacatagt gccaagcaga gatgcagaca
      481 tgtgtacctc taaatcttgt atcactcacc agaagtttgc tatgactctg tatgaacagt
      541 gtgtgtgtcg tagctgtgga gcatcgtcag atcctctacc ttttacagaa tttgtgcggt
      601 acatttctac aacagcctta tgcaatgagg ttgaaagaat gttggaaagg catgaacgct
      661 ttaaacctga aatgtttgca gaattgctac aagcagc
//
