LOCUS       sequence-284070-        2522 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1817
FEATURES             Location/Qualifiers
     source          1..2522
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggggtttt tgctgtccct cccctcagcg ccgacaccat gtggatccag
       61 gttcggacca tggacgggag gcagacccac acggtggact cgctgtccag gctgaccaag
      121 gtggaggagc tgaggcggaa gatccaggag ctgttccacg tggagccagg cctgcagagg
      181 ctgttctaca ggggcaaaca gatggaggac ggccataccc tcttcgacta cgaggtccgc
      241 ctgaatgaca ccatccagct cctggtccgc cagagcctcg tgctccccca cagcaccaag
      301 gagcgggact ccgagctctc cgacaccgac tccggctgct gcctgggcca gagtgagtca
      361 gacaagtcct ccacccacgg tgaggcggcc gccgagactg acagcaggcc agccgatgag
      421 gacatgtggg atgagacgga attggggctg tacaaggtca atgagtacgt cgatgctcgg
      481 gacacgaaca tgggggcgtg gtttgaggcg caggtggtca gggtgacgcg gaaggccccc
      541 tcccgggacg agccctgcag ctccacgtcc aggccggcgc tggaggagga cgtcatttac
      601 cacgtgaaat acgacgacta cccggagaac ggcgtggtcc agatgaactc cagggacgtc
      661 cgagcgcgcg cccgcaccat catcaagtgg caggacctgg aggtgggcca ggtggtcatg
      721 ctcaactaca accccgacaa ccccaaggag cggggcttct ggtacgacgc ggagatctcc
      781 aggaagcgcg agaccaggac ggcgcgggaa ctctacgcca acgtggtgct gggggatgat
      841 tctctgaacg actgtcggat catcttcgtg gacgaagtct tcaagattga gcggccgggt
      901 gaagggagcc ccatggttga caaccccatg agacggaaga gcgggccgtc ctgcaagcac
      961 tgcaaggacg acgtgaacag actctgccgg gtctgcgcct gccacctgtg cgggggccgg
     1021 caggaccccg acaagcagct catgtgcgat gagtgcgaca tggccttcca catctactgc
     1081 ctggacccgc ccctcagcag tgttcccagc gaggacgagt ggtactgccc tgagtgccgg
     1141 aatgatgcca gcgaggtggt actggcggga gagcggctga gagagagcaa gaagaaggcg
     1201 aagatggcct cggccacatc gtcctcacag cgggactggg gcaagggcat ggcctgtgtg
     1261 ggccgcacca aggaatgtac catcgtcccg tccaaccact acggacccat cccggggatc
     1321 cccgtgggca ccatgtggcg gttccgagtc caggtcagcg agtcgggtgt ccatcggccc
     1381 cacgtggctg gcatacacgg ccggagcaac gacggagcgt actccctagt cctggcgggg
     1441 ggctatgagg atgatgtgga ccatgggaat tttttcacat acacgggtag tggtggtcga
     1501 gatctttccg gcaacaagag gaccgcggaa cagtcttgtg atcagaaact caccaacacc
     1561 aacagggcgc tggctctcaa ctgctttgct cccatcaatg accaagaagg ggccgaggcc
     1621 aaggactggc ggtcggggaa gccggtcagg gtggtgcgca atgtcaaggg tggcaagaat
     1681 agcaagtacg cccccgctga gggcaaccgc tacgatggca tctacaaggt tgtgaaatac
     1741 tggcccgaga aggggaagtc cgggtttctc gtgtggcgct accttctgcg gagggacgat
     1801 gatgagcctg gcccttggac gaaggagggg aaggaccgga tcaagaagct ggggctgacc
     1861 atgcagtatc cagaaggcta cctggaagcc ctggccaacc gagagcgaga gaaggagaac
     1921 agcaagaggg aggaggagga gcagcaggag gggggcttcg cgtcccccag gacgggcaag
     1981 ggcaagtgga agcggaagtc ggcaggaggt ggcccgagca gggccgggtc cccgcgccgg
     2041 acatccaaga aaaccaaggt ggagccctac agtctcacgg cccagcagag cagcctcatc
     2101 agagaggaca agagcaacgc caagctgtgg aatgaggtcc tggcgtcact caaggaccgg
     2161 ccggcgagcg gcagcccgtt ccagttgttc ctgagtaaag tggaggagac gttccagtgt
     2221 atctgctgtc aggagctggt gttccggccc atcacgaccg tgtgccagca caacgtgtgc
     2281 aaggactgcc tggacagatc ctttcgggca caggtgttca gctgccctgc ctgccgctac
     2341 gacctgggcc gcagctatgc catgcaggtg aaccagcctc tgcagaccgt cctcaaccag
     2401 ctcttccccg gctacggcaa tggccggtag taatgaacta gtccgtaact tgaaagtatt
     2461 tcgatttctt ggctttatat atcttgtgga aaggacgaga tagctcagca ctctgagttt
     2521 ac
//
