LOCUS       sequence-284082-        4652 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1959
FEATURES             Location/Qualifiers
     source          1..4652
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggccaa tgtgggatcg atgtttcaat attggaagcg ctttgattta
       61 cagcagctgc agagagaact cgatgccacc gcaacggtat tggcgaaccg gcaggatgaa
      121 agtgagcagt ccagaaagcg gcttatcgaa cagagccggg agttcaagaa gaacactcca
      181 gaggatttgc gcaagcaggt agcgccgctg ctgaagagtt tccaaggaga gattgatgca
      241 ctgagtaaaa gaagcaagga agctgaagca gctttcttga atgtctacaa aagattgatt
      301 gacgtcccag atcccgtacc agctttggat ctcggacagc aactccagct caaagtgcag
      361 cgcctgcacg atattgaaac agagaaccag aaacttaggg aaactctgga agaatacaac
      421 aaggaatttg ctgaagtgaa aaatcaagag gttacgataa aagcacttaa agagaaaatc
      481 cgagaatatg aacagacact gaagaaccaa gccgaaacca tagctcttga gaaggaacag
      541 aagttacaga atgactttgc agaaaaggag agaaagctgc aggagacaca gatgtccacc
      601 acctcaaagc tggaggaagc tgagcataag gttcagagcc tacaaacagc cctggaaaaa
      661 actcgaacag aattatttga cctgaaaacc aaatacgatg aagaaactac tgcaaaggcc
      721 gacgagattg aaatgatcat gacggacctt gaaagggcaa accagagggc agaggtggct
      781 cagagagagg cggagacctt aagggaacag ctctcatcgg ccaatcactc cctccagctg
      841 gcctcacaga tccagaaggc accagacgtg gagcaggcca tagaggtgct gacccgctcc
      901 agcctagaag ttgagttggc cgccaaggag cgggagatcg cacagctggt ggaggacgtg
      961 cagagactcc aggccagcct caccaagctg cgggagaatt cggccagcca gatctcacag
     1021 cttgagcagc agctgagcgc caaaaacagc acactcaaac aactggaaga aaaactcaaa
     1081 ggccaggctg actatgaaga ggtgaagaaa gagctgaaca ttctgaagtc catggagttt
     1141 gcaccgtccg agggcgctgg gacacaggat gcggccaagc ccctggaggt gctgttgctg
     1201 gagaagaacc gctcgctgca gtccgagaac gccgcgctgc gcatctccaa cagcgacctg
     1261 agcgggtcag ccaggaggaa agggaaagac cagcctgaaa gtcggcgccc gggatctttg
     1321 ccggcccccc ctccttctca gttgccccgc aacccggggg agcaggcttc caatactaat
     1381 ggtacacacc agttctcacc agcggggtta agtcaagact ttttcagctc atccctggca
     1441 agccccagcc tacccctggc ttctacagga aaatttgcac taaactctct tctccagcgg
     1501 cagctaatgc agtccttcta ctccaaggct atgcaggaag ccggaagcac aagcatgatt
     1561 ttttcaacag gtccatacag cacaaactcc atatcttccc aaagtccatt acaacaaagc
     1621 ccagatgtca atggcatggc cccatccccc agccagtcag aaagtgctgg gagcgtctcc
     1681 gagggcgagg agatggacac tgcagaaatc gcccggcagg tcaaagagca gctgattaag
     1741 cacaatatcg gacaacgtat tttcggacat tatgtgttgg gactgtctca agggtccgtg
     1801 agcgagattc tggcccggcc caagccatgg aataaactga ctgttcgtgg caaggagcca
     1861 tttcacaaga tgaaacagtt cctctccgat gagcagaaca tcctggccct ccgtagcatc
     1921 caaggcagac aaagagagaa tccaggccag agcctgaaca gactatttca ggaagtaccg
     1981 aaacgaagaa atgggtctga aggtaacatc accacccgga tccgagcctc ggagactggc
     2041 tctgatgaag ccatcaagtc catcctagag caagccaaga gggagctcca agtgcagaaa
     2101 actgcagagc cggcccagcc ttcctccgca tccggcagcg ggaactctga tgacgccatc
     2161 cgctccatcc tgcagcaagc ccgccgggag atggaggccc agcaggctgc cctcgaccct
     2221 gccttaaagc aggcaccact gtcccagagt gacatcacca tcctcacccc caagcttctg
     2281 tccacctcgc ccatgcccac cgtgtccagc tacccacctc tcgccatctc cctgaagaag
     2341 ccctccgcag ctcctgaggc cggtgcctct gctctgccga accccccggc cctcaaaaag
     2401 gaggcccagg acgcccccgg gctggacccc cagggagcag ccgattgtgc acaaggggtc
     2461 ctgagacagg tgaaaaatga ggtgggccgc agcggtgcct ggaaggacca ctggtggagc
     2521 gcggtgcagc cggagagaag aaatgccgcc tcctccgagg aggccaaggc cgaagaaacg
     2581 ggcggcggga aagagaaggg cagcggtggc agcggaggtg gcagccagcc tcgggccgag
     2641 cgcagtcagc tccagggacc ctcgtcgtca gagtactgga aggagtggcc cagcgctgag
     2701 tccccatact cccagagctc agagctgagt ctgaccgggg ccagccgcag cgagacacca
     2761 cagaacagcc ccctgccatc ctccccgatc gtgcccatgt ccaagcccac caagccctcg
     2821 gtccccccgc tgacccccga gcagtacgag gtctacatgt accaggaggt ggacaccatc
     2881 gagctcaccc ggcaggttaa ggaaaagctg gccaagaacg gcatctgcca gagaatcttc
     2941 ggggagaagg tgctgggcct gtcccagggc agcgtcagcg acatgctgtc ccgaccgaag
     3001 ccatggagca agctgacgca gaaaggccga gaacccttca tccggatgca gctctggctg
     3061 aacggcgagc taggccaggg tgttctaccc gtccagggcc agcagcaagg gccagtcctc
     3121 cactccgtga catcgctcca ggacccgctg cagcagggct gtgtgagctc agaaagcact
     3181 ccaaagacct ccgccagctg cagccctgcc cctgagtccc cgatgagttc cagtgagtcg
     3241 gtgaagagcc tgaccgagct ggtccagcag ccctgtcccc ccatcgaggc gagcaaggac
     3301 agcaagccac cagagcccag tgacccgcca gcatccgact cccagcccac aaccccgctg
     3361 cctctctccg gacactcggc cctcagcatc caagaattag tagccatgtc cccggagctg
     3421 gacacctacg gcataaccaa gcgggtgaag gaggtgctga cggacaacaa cctcggccag
     3481 cgcttatttg gggagaccat cttagggctc acccaaggct ctgtctctga cctccttgcc
     3541 cgccccaaac cctggcataa gctcagtctg aaaggacgag agcccttcgt ccggatgcag
     3601 ctgtggctga acgaccccaa caatgtggag aagctgatgg acatgaaacg gatggagaag
     3661 aaagcctaca tgaagcggcg gcacagctca gtcagtgaca gccagccctg cgaaccgccc
     3721 tctgtcggca ccgagtacag ccagggcgcc agcccccagc cccagcacca gctgaagaaa
     3781 ccccgggtgg tgctggctcc ggaggagaag gaggcgctga aacgagcgta tcagcaaaag
     3841 ccatacccgt caccaaaaac catcgaagac ctcgccaccc agctcaacct gaaaaccagc
     3901 accgtcatca actggttcca caactacagg tctcggatcc gcagagaact gttcattgag
     3961 gaaattcagg ccgggagtca gggccaggcg ggcgccagcg actcaccctc ggcccgcagc
     4021 ggccgggcgg cgcccagctc ggagggcgac agctgcgacg gcgtggaggc cactgagggc
     4081 ccaggcagcg ccgacaccga ggagcccaag tctcagggag aggccgagcg ggaggaggtg
     4141 ccgcggccgg cggagcagac ggagccgccg ccctcgggga ccccgggccc ggacgacgcc
     4201 cgcgacgacg accacgaggg aggccccgtg gaaggcccgg ggcccctgcc cagccccgcc
     4261 tccgcgaccg ccaccgccgc gcccgcggcc cccgaggacg ccgctacctc agccgccgcc
     4321 gcgccggggg agggccccgc ggccccgagc tccgcgccgc cgcccagcaa cagcagcagc
     4381 agcagcgccc cccgcaggcc cagctcgctg cagagccttt tcggcctccc cgaggccgcg
     4441 ggcgcccggg actcgcgcga caaccccctg cgcaagaaga aggccgcgaa cttgaacagc
     4501 atcatccacc gcctggagaa ggccgccagc cgggaggaac ctatcgaatg ggagttctag
     4561 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     4621 aaggacgaac cggaccaatc gtcggtaagg at
//
