LOCUS       sequence-28409-e         648 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP8
FEATURES             Location/Qualifiers
     source          1..648
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    4..22
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    25..43
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      150..159
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             159..182
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             195..221
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    237..261
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctctccctat cagtgataga gatctcccta tcagtgatag agatcgtcga cgagctcgtt
       61 nagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
      121 accgggaccg atccagcctc cggggatctg ccaccatgga ctacaaggat gacgatgaca
      181 agctcgatgg aggataccca tacgatgttc cagattacgc tgctcgaggt tatcaaacaa
      241 gtttgtacaa aaaagttggc atgcctgctg tggcttcagt tcctaaagaa ctctacctca
      301 gttcttcact aaaagacctt aataagaaga cagaagttaa accagagaaa ataagcacta
      361 agagttatgt gcacagtgcc ctgaagatct ttaagacagc agaagaatgc agattagatc
      421 gtgatgagga aagggcctat gtactatata tgaaatacgt gactgtttat aatcttatca
      481 aaaaaagacc tgatttcaag caacagcagg attatttcca ttcaatactt ggacctggaa
      541 acatcaaaaa agctgtnnaa gaagctgaaa gactctctga aagccttaaa ttaagatatg
      601 aagaagctga agtccggaaa aaacttgagg aaaaagacag gcaggagg
//
