LOCUS       sequence-284094-        4004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1988
FEATURES             Location/Qualifiers
     source          1..4004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaagaagc gagcctgtgc cttggagtgt cttcggcgga gccggaagct
       61 gagccccacc tgagtggccc cgtcctcaac ggccagtatg ccatgagtca gaagctgcac
      121 cagatcacct cccagctcag ccatgccttc cccgagctcc atccccggcc caaccccgag
      181 gagaagcccc ccgcatccct ggaggagaag gcccacgtgc ccatgagcgg ccagcccatg
      241 ggcagtcaga tggcgctcct ggccaaccag ctgggccggg aggtggacac cagcctcaac
      301 gggagggtgg acttgcagca gttcctcaac gggcagaacc tgggcatcat gtcccagatg
      361 agcgacatcg aggacgacgc ccgcaagaac cgcaagtacc cgtgcccact ctgcggcaag
      421 cgcttccgct tcaacagcat cctctccctg cacatgcgca cgcacacggg cgagaagccc
      481 ttcaagtgcc cgtactgcga ccacagggcg gcgcagaagg ggaacctcaa gattcacctg
      541 cggacccaca agctgggcaa cctgggcaag gggcgtgggc gtgtgcgcga ggagaaccgc
      601 ctgctgcacg agctggagga gcgcgccatc ctgcgggaca agcagctgaa aggcagcctg
      661 ctgcagcccc ggccggacct gaagcccccg ccgcacgccc agcaggcccc gctggccgcc
      721 tgcaccctgg ccctgcaggc taaccacagc gttcccgacg tggcccaccc ggtgccctcg
      781 cccaagcctg ccagcgtgca ggaggacgcg gtggccccgg cggcgggctt ccgctgtacc
      841 ttctgcaagg gcaagttcaa gaagcgcgag gagctggacc gccacatccg catcttgcac
      901 aagccctaca agtgcacgtt gtgcgacttc gcggcttcgc aggaggagga gctcatcagc
      961 cacgtggaga aggcacacat cacggccgag tcggcccagg gccagggccc caacggcggt
     1021 ggcgagcagt cggccaacga gttccgctgc gaggtgtgcg gtcaggtgtt cagccaggcg
     1081 tggttcctca agggtcacat gcgcaagcac aaagactcct ttgagcactg ctgccagatc
     1141 tgcggccggc gcttcaagga gccctggttc ctcaagaacc acatgaaggt ccacctcaac
     1201 aagctgtcgg tgaagaacaa gtcccccagc gaccccgagg tgcctgtgcc catgggcggc
     1261 atgtcccagg aggcccacgc caacctgtac tccaggtacc tctcctgcct gcagagtggc
     1321 ttcatgaccc cggacaaagc cggcctgagc gagcccagcc agctctatgg caagggcgag
     1381 ctgcccatga aggagaagga agcgctgggg aagctgctgt ctcccatctc cagcatggcc
     1441 cacggcgtcc cggaggggga caagcactcc ctcctgggat gcctcaatct cgtgccgccg
     1501 ctgaaatcca gctgcatcga gcggctgcag gcggctgcca aggctgcgga gatggacccc
     1561 gtgaacagct accaggcttg gcagctcatg gccaggggca tggccatgga acatggcttc
     1621 ttgtctaaag agcatccgct gcagcgcaac cacgaagaca ctttggcaaa cgccggggtt
     1681 ctgtttgata aggagaagcg ggagtacgtg ttagtgggag cagatggctc caagcagaaa
     1741 atgcctgctg atttggttca cagcactaaa gtgggcagcc agagagacct gccaagtaag
     1801 ctcgaccctt tagaaagcag tcgggatttt ttgtcacacg ggctgaacca gactctcgag
     1861 tataacctgc agggtcctgg gaacatgaag gagaagccca ccgagtgccc cgactgcggc
     1921 cgggtgttcc gcacttacca ccaggtggtc gtgcactccc gtgtccacaa gcgggaccgc
     1981 aagggcgagg aggatgggct gcacgtgggc ctggatgagc ggcgtggctc gggcagtgac
     2041 caggagtccc agtcggtgag ccgctccacc acgccgggct cctctaacgt caccgaggag
     2101 agcggggtcg gaggcggcct ctcccagacc gggagtgccc aggaggacag cccgcacccc
     2161 tcctcgccat cctcctcaga cattggcgag gaggctggga gatctgccgg cgtccagcaa
     2221 ccagcgctgc ttcgcgacag aagcctgggc tcggccatga aggactgccc gtactgtggg
     2281 aaaactttcc ggacatccca tcaccttaag gtgcacctga ggatacacac aggtgagaaa
     2341 ccctacaagt gtccgcactg tgactatgcc ggcacgcagt cagcatcctt aaaataccac
     2401 ttagagcgac accatcggga gcggcagaac ggggctgggc cgctgtctgg gcaaccccca
     2461 aatcaagacc acaaggatga gatgtcaagc aaagcttctc tgttcatcag gccagacatc
     2521 ctgagggggg ccttcaaggg tctccctgga atcgacttca gaggaggccc tgcatctcag
     2581 cagtggacat caggggttct ctcctctgga gatcactcgg ggcaggccac gggcatgtct
     2641 tcggaggtcc cctcagatgc tctgaaaggc actgaccttc cttccaaaag cacccacttc
     2701 tctgagatcg gaagagctta tcaaagcatt gtgagcaacg gtgtgaattt ccaagggtcc
     2761 ttgcaagctt tcatggacag ttttgtcctc agttccttga agaaggagaa ggacatgaag
     2821 gacaaagccc tggctgaccc cccttccatg aaagtccacg gagtggatgg tggtgaggag
     2881 aaacccagtg gcaagtcctc ccagaggaag tccgagaaat ctcagtatga acccctggac
     2941 ttgtctgtgc ggccagatgc cgcctccctc ccgggctcct cggtaactgt gcaggacagc
     3001 attgcatggc acggctgctt gttttgtgct ttcacaacgt cctccatgga gctcatggcc
     3061 cttcatctcc aggccaacca cctgggcaaa gcgaaacgca aagataacac catcggggtc
     3121 acagtcaact gcaaagacca agcccgggag gcgagtaaga tggccctgct gccctcgtta
     3181 caatcaaaca aagacctggg cctctccaat atgatcagct ctctagactc tgcttctgag
     3241 aagatggccc aaggtcagct caaggagact ctgggagagc agaagagcgg tgcatggacc
     3301 ggccacgtgg accctgcatt ttgtaacttc ccatcagact tctacaagca gtttggtgtt
     3361 tacccaggca tggttggctc aggggcctcc agttcctgcc ccaacaagga gcctgatgga
     3421 aaggcccact ctgaagagga tgtccccatc ctgatccccg aaaccacgag taagaacact
     3481 actgatgacc tctctgacat tgcctcctca gaggacatgg actcctccaa gggggagaac
     3541 aacgatgaag aggatgttga aaccgaaccg gaaatgatga ccaagccact gtctgccctc
     3601 agcaaagaca gcagcagcga tggcggggac agcctgcagc ccacaggcac ctcccagccc
     3661 gtccagggac tggtctcacc tttatcccaa gcaccggaga agcagtggca cagccagggt
     3721 cttctccaag cccaggaccc cttggcgggc ctgccaaagc cggagcgggg gccccagagc
     3781 ctggacaagc cgatgaacat gctgtcggtc ctcagggcct acagttctga tggcttagca
     3841 gcctttaacg gacttgcaag tagcacagca aattctggat gtatcaagag gccagacttg
     3901 tgtggtaagt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     3961 atatcttgtg gaaaggacga gaggcgatcg cagcatgcca gatc
//
