LOCUS       sequence-284097-        2972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1992
FEATURES             Location/Qualifiers
     source          1..2972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccagcaa gaggaaatcc accacaccat gcatgatccc agtgaagact
       61 gtggtgttgc aagatgccag catggaggcc cagcccgctg agaccttgcc tgaaggaccc
      121 cagcaggatc tgcccccaga agcatctgct gccagcagtg aggcagcaca gaaccccagc
      181 agtactgatg gctctacact ggccaatggg catcggagca ctttagatgg ctatttatat
      241 tcctgtaaat actgcgattt cagatcccat gacatgaccc aatttgtggg acatatgaac
      301 tcagagcaca cagactttaa taaagaccca acctttgtat gcagtgggtg cagttttctg
      361 gcaaaaaccc ctgaggggct ttccttgcac aatgccacat gtcactccgg ggaagccagc
      421 tttgtgtgga acgtggccaa gccagacaat catgtggttg tggagcagag catccctgag
      481 agcaccagca ctcctgacct agcgggtgag cccagtgctg aaggggctga tggacaggca
      541 gaaatcatca ttaccaaaac tccaatcatg aagataatga aaggcaaagc tgaagccaaa
      601 aaaattcata cactcaagga gaatgtccct agccagcctg tgggtgaggc cttaccaaag
      661 ctgtcgactg gagaaatgga ggtgagagag ggggaccatt ccttcatcaa tggggcagtt
      721 ccagtcagcc aggcatctgc cagctctgca aaaaaccccc atgccgccaa cgggcccctg
      781 ataggaacag tgccagtttt gccagctggc atagcacagt tcctctccct ccagcagcag
      841 cccccagtgc atgcccaaca ccatgtccac cagccactgc ccacggccaa ggcccttccc
      901 aaagtgatga tccccctgag cagcattcca acgtacaatg cagccatgga ctctaacagc
      961 ttcctgaaga actccttcca caagttcccc taccccacca aagccgagct ctgctatttg
     1021 actgtggtga ccaagtatcc agaagaacag ctcaagatct ggttcacagc ccaaaggctg
     1081 aagcagggga tcagctggtc ccctgaggag attgaggatg cccggaaaaa gatgttcaat
     1141 acagtcatcc agtctgtgcc tcagcccaca attacggttc taaatacccc actcgtcgcc
     1201 agtgctggca atgtccagca tctcatccag gccgctcttc caggtcacgt tgtggggcag
     1261 ccagagggta caggaggggg acttctggtc actcagccat tgatggccaa tgggttgcaa
     1321 gcaacaagtt cccctctccc cctcacggtg acatccgtcc ccaagcagcc aggtgtggca
     1381 cccattaaca ctgtgtgttc aaatacaacg tcagctgtga aggtggtcaa tgcggcccag
     1441 tcgctcctca cggcctgccc cagcataacc tcccaagcct tccttgatgc tagcatctac
     1501 aaaaataaga aatctcatga acagctgtca gctctgaaag ggagcttctg tcggaaccag
     1561 ttcccagggc agagcgaagt tgaacatctc acaaaagtga cgggcctcag taccagagag
     1621 gtgcggaaat ggttcagtga tcgtagatac cactgccgga acttgaaggg ctccagagcg
     1681 atgatacctg gagatcacag ttccatcatc attgactctg tgccagaggt gtccttctcc
     1741 ccatcgtcca aggtccctga ggtaacctgc attccgacaa cagccacact agcaacccac
     1801 ccttctgcca aacgacaatc ttggcaccag actcctgact tcacaccaac caaatacaag
     1861 gagagagccc ctgagcagct cagagccctg gagagcagtt ttgcacaaaa ccctcttcct
     1921 cttgatgagg aactggaccg cctgagaagt gaaaccaaaa tgacccgacg agaaattgat
     1981 agctggtttt cagagagacg gaaaaaagtg aatgctgagg agaccaagaa ggctgaggag
     2041 aatgcctctc aggaggaaga ggaggctgct gaggatgagg gtggagaaga ggatttggcc
     2101 agtgagctaa gggtctctgg tgaaaatggc tctctggaaa tgcccagcag ccatatcttg
     2161 gcagagcgca aagtcagccc cattaaaatc aacctgaaga acctgagggt cactgaagcc
     2221 aatggcagga acgagattcc agggctgggt gcctgtgacc ctgaggatga tgagtcaaac
     2281 aaactggcag agcagctccc aggcaaagtg agctgcaaaa agactgccca gcagcggcac
     2341 ttgctgcggc agctctttgt ccagacacag tggccaagca accaggacta tgactccatc
     2401 atggcccaga cgggtctgcc acggccagag gtggtgcgct ggtttggaga tagcaggtac
     2461 gcactgaaga acggccaact caaatggtac gaagactata agcgaggcaa cttcccacca
     2521 gggctactgg tcattgcccc tggcaaccgg gagctcctgc aggactatta catgacacac
     2581 aagatgctgt atgaagagga cctgcagaac ctctgtgaca agacccagat gagctcccag
     2641 caggtcaagc agtggtttgc tgagaaaatg ggggaggaga ccagagccgt ggcagacaca
     2701 ggcagtgagg accagggccc tggtactggt gagctcacag cagttcacaa agggatgggt
     2761 gacacctatt cagaggtgtc tgagaacagt gagtcgtggg agccccgtgt ccctgaggcc
     2821 agctcagagc cctttgacac atcgagtccc caggctggac gtcagctcga aacagactag
     2881 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     2941 aaggacgacc acgccttgaa gttctagcct gc
//
