LOCUS       sequence-284100-        1904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2000
FEATURES             Location/Qualifiers
     source          1..1904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggacgc caacaaggcc gaggtgcccg gggccactgg tggcgacagc
       61 ccgcacctgc agcccgcaga gccgccgggc gagccgcggc gagagccgca ccccgcggag
      121 gcggagaagc agcagccgca gcacagcagc agctccaatg gcgttaaaat ggagaatgat
      181 gaatcagcaa aagaagagaa atctgactta aaggaaaaat ctacaggaag taagaaggcc
      241 aatagatttc atccttattc aaaagacaag aattcgggcg ctggagaaaa gaagggtcca
      301 aatcgtaaca gagttttcat tagcaacatc ccatatgaca tgaaatggca agctattaaa
      361 gatctaatga gagagaaagt tggtgaggtt acatacgtgg agctctttaa ggatgcggaa
      421 ggaaaatcaa ggggttgtgg tgtggttgaa ttcaaagatg aagaatttgt aaagaaagcc
      481 ctagaaacta tgaacaaata tgatcttagt ggaagacccc ttaatattaa agaggatcct
      541 gatggagaaa atgctcgtag ggcattgcag cgaacaggag gatcatttcc aggaggacac
      601 gtccctgata tgggatcagg gttgatgaat ttaccacctt ccatactcaa taatccaaac
      661 attcctcctg aagtcatcag taatttgcag gccggtagac ttggttccac aatttttgtt
      721 gccaatcttg acttcaaagt tggttggaag aagctaaagg aagtgttcag catagctgga
      781 actgtgaagc gggcagatat taaagaagac aaagatggca agagcagagg aatgggcact
      841 gtcacttttg agcaagcaat tgaagcagtt caagcaattt ctatgttcaa tgggcagttt
      901 ttatttgata gacctatgca tgtgaaaatg gatgacaagt ctgttcctca tgaagagtac
      961 cgttcacatg atggtaaaac accacaatta ccacgtggtc ttggaggcat tgggatggga
     1021 cttggtccgg gtggacagcc tattagtgcc agccagttga acataggtgg agtaatggga
     1081 aatttaggtc caggtggtat gggaatggat ggtccaggtt ttggaggaat gaatagaatt
     1141 ggaggaggaa tagggtttgg tggtctggaa gcaatgaata gcatgggagg atttggagga
     1201 gttggccgaa tgggagagct gtaccgtggt gcgatgacta gtagcatgga gcgagatttt
     1261 ggacgtggtg atattggaat aaatcgaggc tttggagatt cctttggtag acttggcagt
     1321 gcaatgattg gagggtttgc aggaagaata ggatcttcta acatgggtcc agtaggatct
     1381 ggaataagtg gtggaatggg tagcatgaac agtgtgactg gaggaatggg gatgggactg
     1441 gaccggatga gttccagctt tgatagaatg ggaccaggta taggagctat actggaaagg
     1501 agcatcgata tggatcgagg atttttatcg ggtccaatgg gaagcggaat gagagagaga
     1561 ataggctcca aaggcaacca gatatttgtc agaaatctac cttttgactt gacttggcag
     1621 aaactaaaag agaaattcag tcagtgtggt catgtaatgt ttgcagaaat aaaaatggag
     1681 aatggaaagt caaaaggctg tggaacagtc agatttgact ccccagaatc agctgaaaaa
     1741 gcctgcagaa taatgaatgg cataaaaatc agtggcagag aaattgatgt tcgcttggat
     1801 cgtaatgcat agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1861 atatcttgtg gaaaggacga gtgctgagca gtgcctccca atat
//
