LOCUS       sequence-284106-        2576 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2027
FEATURES             Location/Qualifiers
     source          1..2576
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatgaaga tgagaaagac agagccaaga gagcttctcg aaacaagtct
       61 gagaagaagc gtcgggacca gttcaatgtt ctcatcaaag agctcagttc catgctccct
      121 ggcaacacgc ggaaaatgga caaaaccacc gtgttggaaa aggtcatcgg atttttgcag
      181 aaacacaatg aagtctcagc gcaaacggaa atctgtgaca ttcagcaaga ctggaagcct
      241 tcattcctca gtaatgaaga attcacccag ctgatgttgg aggcattaga tggcttcatt
      301 atcgcagtga caacagacgg cagcatcatc tatgtctctg acagtatcac gcctctcctt
      361 gggcatttac cgtcggatgt catggatcag aatttgttaa atttcctccc agaacaagaa
      421 cattcagaag tttataaaat cctttcttcc catatgcttg tgacggattc cccctcccca
      481 gaatacttaa aatctgacag cgatttagag ttttattgcc atcttctcag aggcagcttg
      541 aacccaaagg aatttccaac ttatgaatac ataaaatttg taggaaattt tcgctcttac
      601 aacaatgtgc ctagcccctc ctgtaatggt tttgacaaca ccctttcaag accttgccgg
      661 gtgccactag gaaaggaggt ttgcttcatt gccaccgttc gtctggcaac accacaattc
      721 ttaaaggaaa tgtgcatagt tgacgaacct ttagaggaat tcacttcaag gcatagcttg
      781 gaatggaaat ttttatttct ggatcacaga gcacctccaa tcataggata cctgcctttt
      841 gaagtgctgg gaacctcagg ctatgactac taccacattg atgacctgga gctcctggcc
      901 aggtgtcacc agcacctgat gcagtttggc aaagggaagt cgtgttgcta ccggtttctg
      961 accaaaggtc agcagtggat ctggctgcag actcactact acatcaccta ccatcagtgg
     1021 aactccaagc ccgagttcat cgtgtgcaca cactcggtgg tcagttacgc agatgtccgg
     1081 gtggaaagga ggcaggagct ggctctggaa gacccgccat ccgaggccct ccactcctca
     1141 gcactaaagg acaagggctc aagcctggaa cctcggcagc actttaacac actcgacgtg
     1201 ggtgcctcgg gccttaatac cagtcattcg ccatcggcgt cctcaagaag ttcccacaaa
     1261 tcctcgcaca cagccatgtc agaacccacc tccactccca ccaagctgat ggcagaggcc
     1321 agcaccccgg ctttgccaag atcagccacc ctgccccaag agttacctgt ccccgggctc
     1381 agccaggcag ccaccatgcc ggcccctctg ccttccccat cgtcctgcga cctcacacag
     1441 cagctcctgc ctcagaccgt tctgcagagc acgcccgctc ccatggcaca gttttcggca
     1501 cagttcagca tgttccagac catcaaagac cagctagagc agcggacgcg gatcctgcag
     1561 gccaatatcc ggtggcaaca ggaagagctc cacaagatcc aggagcagct ctgcctggtc
     1621 caggactcca acgtccagat gttcctgcag cagccagctg tatccctgag cttcagcagc
     1681 acccagcgac ctgaggctca gcagcagcta cagcaaaggt cagctgcagt gactcagccc
     1741 cagctcgggg cgggccccca acttccaggg cagatctcct ctgcccaggt cacaagccag
     1801 cacctgctca gagaatcaag tgtgatatca acccagggtc caaagccaat gagaagctca
     1861 cagctaatgc agagcagcgg ccgctctgga agcagcctag tgtccccgtt cagcagcgcc
     1921 acagctgcgc tcccgccaag tctgaatctg accacacctg cttccacctc ccaggatgcc
     1981 agccagtgcc agcccagccc agacttcagc catgatcggc agctcaggct gttgctgagc
     2041 cagcccatcc agcccatgat gcccgggtcc tgtgacgcaa ggcagccctc ggaagtcagc
     2101 aggacgggac ggcaagtcaa gtacgcccag agccagaccg tgtttcaaaa tccagacgca
     2161 caccccgcca acagcagcag cgccccgatg cccgtcctgc tgatggggca ggcggtgctc
     2221 caccccagct tccctgcctc ccaaccatcg cccctgcagc ctgcacaggc ccggcagcag
     2281 ccaccgcagc actacctgca ggtacaggca ccaacctctt tgcacagtga gcagcaggac
     2341 tcgctacttc tctccaccta ctcacaacag ccagggaccc tgggctaccc ccaaccaccc
     2401 ccagcacagc cccagcccct acgtcctccc cgaagggtca gcagtctgtc tgagtcgtca
     2461 ggcctccagc agccgccccg atagtaatga actagtccgt aacttgaaag tatttcgatt
     2521 tcttggcttt atatatcttg tggaaaggac gaggactaag agaggttccg atcgaa
//
