LOCUS       sequence-284112-        2204 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2051
FEATURES             Location/Qualifiers
     source          1..2204
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggagct gatgctgctc agcgagattg ctgacccgac gcgtttcttc
       61 accgacaacc tgcttagccc ggaggactgg gacagcacct tgtattctgg cctagatgaa
      121 gtggccgagg agcagacgca gctcttccgt tgcccggagc aggatgtccc gtttgacggc
      181 agctccctgg acgtggggat ggatgtcagc ccctctgagc ccccatggga actcctgccg
      241 atcttcccag atcttcaggt gaagtctgag ccatcttccc cctgctcttc ctcctccctc
      301 agctccgagt catcgcgtct ctccacagag ccatccagcg aggctcttgg ggtaggggag
      361 gtgctccatg tgaagacaga gtccttggca cccccactgt gtctcctggg agatgaccca
      421 acatcctcat ttgaaaccgt ccagatcaac gttatcccca cctctgatga ttcctcagat
      481 gtccagacca agatagaacc tgtctctcca tgttcttccg tcaactctga ggcctccctg
      541 ctctcagccg actcctccag ccaggctttt ataggagagg aggtcctgga agtgaagaca
      601 gagtccctgt ccccttcagg atgcctcctg tgggatgtcc cagccccctc acttggagct
      661 gtccagatca gcatgggccc atcccttgat ggctcctcag gcaaagccct gcccacccgg
      721 aagccgccac tgcagcccaa acctgtagtg ctaaccactg tcccaatgcc atccagagct
      781 gtgcctccca gcaccacagt ccttctgcag tccctcgtcc agccaccccc agtgtcccca
      841 gttgtcctca tccagggtgc tattcgagtc cagcctgaag ggccggctcc ctctctacca
      901 cggcctgaga ggaagagcat cgttcccgct cctatgcctg gaaactcctg cccgcctgaa
      961 gtggatgcaa agctgctgaa gcggcagcag cgaatgatca agaaccggga gtcagcctgc
     1021 cagtcccgga gaaagaagaa agagtatctg cagggactgg aggctcggct gcaagcagta
     1081 ctggctgaca accagcagct ccgccgagag aatgctgccc tccggcggcg gctggaggcc
     1141 ctgctggctg aaaacagcga gctcaagtta gggtctggaa acaggaaggt ggtctgcatc
     1201 atggtcttcc ttctcttcat tgccttcaac tttggacctg tcagcatcag tgagcctcct
     1261 tcagctccca tctctcctcg gatgaacaag ggggagcctc aaccccggag acacttgctg
     1321 gggttctcag agcaagagcc agttcaggga gttgaacctc tccaggggtc ctcccagggc
     1381 cctaaggagc cccagcccag ccccacagac cagcccagtt tcagcaacct gacagccttc
     1441 cctgggggcg ccaaggagct actactaaga gacctagacc agctcttcct ctcctctgat
     1501 tgccggcact tcaaccgcac tgagtccctg aggcttgctg acgagttgag tggctgggtc
     1561 cagcgccacc agagaggccg gaggaagatc cctcagaggg cccaggagag acagaagtct
     1621 cagccacgga agaagtcacc tccagttaag gcagtcccca tccaaccccc tggaccccca
     1681 gaaagggatt ctgtgggcca gctgcaacta tatcgccacc cagaccgttc gcagccagca
     1741 ttcttggatg caattgaccg acgggaagac acattttatg ttgtctcttt ccgaagggac
     1801 cacctgctgc tcccagccat cagccacaac aagacctccc ggcccaagat gtccctggtg
     1861 atgcctgcca tggcccccaa tgagaccctg tcaggccgtg gggccccggg ggactatgag
     1921 gagatgatgc agatcgagtg tgaggtcatg gacaccaggg tgattcacat caagacctcc
     1981 acagtgcccc cctcgctccg aaaacagcca tccccaaccc caggcaatgc cacaggtggc
     2041 cccttgccag tctctgcagc cagccaggcc caccaggcct cccaccagcc cctctacctc
     2101 aatcatccct agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     2161 atatcttgtg gaaaggacga catgtaatga gtgcttaaat gaaa
//
