LOCUS       sequence-28412-e         679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-GFP
FEATURES             Location/Qualifiers
     source          1..679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     68..90
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     106..122
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      151..160
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             160..183
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             196..222
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    238..262
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(307..328)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(568..587)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tccctgggtc agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg
       61 tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc
      121 actccttctc taggcgccgg aattagatct gccaccatgg actacaagga tgacgatgac
      181 aagctcgatg gaggataccc atacgatgtt ccagattacg ctgctcgagg ttatcaaaca
      241 agtttgtaca aaaaagttgg catggtgagc aagggcgagg agctgttcac cggggtggtg
      301 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      361 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      421 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
      481 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
      541 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
      601 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      661 gacggcaaca tcctgggcc
//
