LOCUS       sequence-284121-        1496 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2173
FEATURES             Location/Qualifiers
     source          1..1496
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggcggc cgccctgagg gaccccgctc agggctatgt gacctttgag
       61 gatgtggctg tctacttctc ccaggaggaa tggagattgc ttgatgacgc tcagaggctc
      121 ctctaccgca atgtgatgct ggagaacttt acacttctgg cctctctggg ttgttggcat
      181 ggagccgagg ctgaggaggc tcctgagcag attgcttctg taggactgct cagttcaaac
      241 attcagcaac accagaagca gcactgtgga gagaaaccct taaaaagaca agagggcagg
      301 gtcccagttt tgaggagttg caaagttcac ctatcagaga agtccttgca aagcagggag
      361 gttgggaagg ccctcctgat cagctcaggt gttctcaagc accaggtgac tcacacagga
      421 gagaagtcac ataggagctc caaaagtagg gaggcctttc atgctggaaa aaggcattac
      481 aaatgcagtg aatgtgggaa agcctttggt cagaaatatt tacttgttca gcaccagaga
      541 ctacatgctg ggaaaaagac gtatgaatgc agtgaatgtg ggaagttatt tagagatatg
      601 tccaaccttt ttatacacca aatagttcac actggagaaa ggccttacgg gtgtagtaac
      661 tgtggaaaat cctttagccg taatgctcac ctcattgaac accagagagt tcacactgga
      721 gaaaagcctt ttacatgcag tgaatgtgga aaagctttca ggcataattc cacacttgtt
      781 cagcatcaca aaatccacac tggagtaagg ccttatgagt gcagtgaatg tggaaaattg
      841 tttagtttca actccagcct catgaaacat cagagaattc acactggaga aagaccttat
      901 aagtgcagtg aatgtggaaa attctatagc cacaagtcca accttatcaa acattggcgt
      961 gttcatactg gagaaaggcc ttacaagtgc agcgactgtg ggaaattttt tacccaatgc
     1021 tcaagcctca tgcaacatca aaaagttcac actggagaaa agccttttaa gtgcaatgaa
     1081 tgtgggagat tctttagaga gaattccacc ctagttagac atcagagggt tcacactgga
     1141 gcaaagcctt atgagtgcag ggaatgtggg aaatttttta gccaaagctc aaccctcatg
     1201 caacatcgaa aagttcacat tggagaaaag ccttttaagt gcaatgaatg tgggagattg
     1261 tttagagaga attccagcct tgttaaacat cagagggttc acactggagc aaagccttat
     1321 gagtgcaggg aatgtgggaa gttttttcgc cacaactcca gtctttttaa acatcgaagg
     1381 attcacactg gagaaatgca gtagtaatga actagtccgt aacttgaaag tatttcgatt
     1441 tcttggcttt atatatcttg tggaaaggac gacgctgtcg taggatggga ccttca
//
