LOCUS       sequence-284124-        1637 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2174
FEATURES             Location/Qualifiers
     source          1..1637
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggcggc cgccctgagg gaccccgctc aggttcctgt ggctgcagac
       61 ttgcttacag accatgagga gcagggctat gtgacctttg aggatgtggc tgtctacttc
      121 tcccaggagg aatggagatt gcttgatgac gctcagaggc tcctctaccg caatgtgatg
      181 ctggagaact ttacacttct ggcctctctg ggacttgcat cttccaagac ccatgaaata
      241 acccagctgg agtcatggga ggagcccttc atgcctgctt gggaagttgt gacttcagcc
      301 ataccgagag gttgttggca tggagccgag gctgaggagg ctcctgagca gattgcttct
      361 gtaggactgc tcagttcaaa cattcagcaa caccagaagc agcactgtgg agagaaaccc
      421 ttaaaaagac aagagggcag ggtcccagtt ttgaggagtt gcaaagttca cctatcagag
      481 aagtccttgc aaagcaggga ggttgggaag gccctcctga tcagctcagg tgttctcaag
      541 caccaggtga ctcacacagg agagaagtca cataggagct ccaaaagtag ggaggccttt
      601 catgctggaa aaaggcatta caaatgcagt gaatgtggga aagcctttgg tcagaaatat
      661 ttacttgttc agcaccagag actacatgct gggaaaaaga cgtatgaatg cagtgaatgt
      721 gggaagttat ttagagatat gtccaacctt tttatacacc aaatagttca cactggagaa
      781 aggccttacg ggtgtagtaa ctgtggaaaa tcctttagcc gtaatgctca cctcattgaa
      841 caccagagag ttcacactgg agaaaagcct tttacatgca gtgaatgtgg aaaagctttc
      901 aggcataatt ccacacttgt tcagcatcac aaaatccaca ctggagtaag gccttatgag
      961 tgcagtgaat gtggaaaatt gtttagtttc aactccagcc tcatgaaaca tcagagaatt
     1021 cacactggag aaagacctta taagtgcagt gaatgtggaa aattctatag ccacaagtcc
     1081 aaccttatca aacattggcg tgttcatact ggagaaaggc cttacaagtg cagcgactgt
     1141 gggaaatttt ttacccaatg ctcaagcctc atgcaacatc aaaaagttca cactggagaa
     1201 aagcctttta agtgcaatga atgtgggaga ttctttagag agaattccac cctagttaga
     1261 catcagaggg ttcacactgg agcaaagcct tatgagtgca gggaatgtgg gaaatttttt
     1321 agccaaagct caaccctcat gcaacatcga aaagttcaca ttggagaaaa gccttttaag
     1381 tgcaatgaat gtgggagatt gtttagagag aattccagcc ttgttaaaca tcagagggtt
     1441 cacactggag caaagcctta tgagtgcagg gaatgtggga agttttttcg ccacaactcc
     1501 agtcttttta aacatcgaag gattcacact ggagaaatgc agtagtaatg aactagtccg
     1561 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaagtaact
     1621 gcgtggagcg agttagc
//
