LOCUS       sequence-284127-        1538 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2175
FEATURES             Location/Qualifiers
     source          1..1538
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggcggc cgccctgagg gaccccgctc aggttcctgt ggctgcagac
       61 ttgcttacag accatgagga gcagggctat gtgacctttg aggatgtggc tgtctacttc
      121 tcccaggagg aatggagatt gcttgatgac gctcagaggc tcctctaccg caatgtgatg
      181 ctggagaact ttacacttct ggcctctctg ggttgttggc atggagccga ggctgaggag
      241 gctcctgagc agattgcttc tgtaggactg ctcagttcaa acattcagca acaccagaag
      301 cagcactgtg gagagaaacc cttaaaaaga caagagggca gggtcccagt tttgaggagt
      361 tgcaaagttc acctatcaga gaagtccttg caaagcaggg aggttgggaa ggccctcctg
      421 atcagctcag gtgttctcaa gcaccaggtg actcacacag gagagaagtc acataggagc
      481 tccaaaagta gggaggcctt tcatgctgga aaaaggcatt acaaatgcag tgaatgtggg
      541 aaagcctttg gtcagaaata tttacttgtt cagcaccaga gactacatgc tgggaaaaag
      601 acgtatgaat gcagtgaatg tgggaagtta tttagagata tgtccaacct ttttatacac
      661 caaatagttc acactggaga aaggccttac gggtgtagta actgtggaaa atcctttagc
      721 cgtaatgctc acctcattga acaccagaga gttcacactg gagaaaagcc ttttacatgc
      781 agtgaatgtg gaaaagcttt caggcataat tccacacttg ttcagcatca caaaatccac
      841 actggagtaa ggccttatga gtgcagtgaa tgtggaaaat tgtttagttt caactccagc
      901 ctcatgaaac atcagagaat tcacactgga gaaagacctt ataagtgcag tgaatgtgga
      961 aaattctata gccacaagtc caaccttatc aaacattggc gtgttcatac tggagaaagg
     1021 ccttacaagt gcagcgactg tgggaaattt tttacccaat gctcaagcct catgcaacat
     1081 caaaaagttc acactggaga aaagcctttt aagtgcaatg aatgtgggag attctttaga
     1141 gagaattcca ccctagttag acatcagagg gttcacactg gagcaaagcc ttatgagtgc
     1201 agggaatgtg ggaaattttt tagccaaagc tcaaccctca tgcaacatcg aaaagttcac
     1261 attggagaaa agccttttaa gtgcaatgaa tgtgggagat tgtttagaga gaattccagc
     1321 cttgttaaac atcagagggt tcacactgga gcaaagcctt atgagtgcag ggaatgtggg
     1381 aagttttttc gccacaactc cagtcttttt aaacatcgaa ggattcacac tggagaaatg
     1441 cagtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1501 tgtggaaagg acgagatctg tgatgcagca atgatctg
//
