LOCUS       sequence-284130-        1598 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2176
FEATURES             Location/Qualifiers
     source          1..1598
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggcggc cgccctgagg gaccccgctc agcagggcta tgtgaccttt
       61 gaggatgtgg ctgtctactt ctcccaggag gaatggagat tgcttgatga cgctcagagg
      121 ctcctctacc gcaatgtgat gctggagaac tttacacttc tggcctctct gggacttgca
      181 tcttccaaga cccatgaaat aacccagctg gagtcatggg aggagccctt catgcctgct
      241 tgggaagttg tgacttcagc cataccgaga ggttgttggc atggagccga ggctgaggag
      301 gctcctgagc agattgcttc tgtaggactg ctcagttcaa acattcagca acaccagaag
      361 cagcactgtg gagagaaacc cttaaaaaga caagagggca gggtcccagt tttgaggagt
      421 tgcaaagttc acctatcaga gaagtccttg caaagcaggg aggttgggaa ggccctcctg
      481 atcagctcag gtgttctcaa gcaccaggtg actcacacag gagagaagtc acataggagc
      541 tccaaaagta gggaggcctt tcatgctgga aaaaggcatt acaaatgcag tgaatgtggg
      601 aaagcctttg gtcagaaata tttacttgtt cagcaccaga gactacatgc tgggaaaaag
      661 acgtatgaat gcagtgaatg tgggaagtta tttagagata tgtccaacct ttttatacac
      721 caaatagttc acactggaga aaggccttac gggtgtagta actgtggaaa atcctttagc
      781 cgtaatgctc acctcattga acaccagaga gttcacactg gagaaaagcc ttttacatgc
      841 agtgaatgtg gaaaagcttt caggcataat tccacacttg ttcagcatca caaaatccac
      901 actggagtaa ggccttatga gtgcagtgaa tgtggaaaat tgtttagttt caactccagc
      961 ctcatgaaac atcagagaat tcacactgga gaaagacctt ataagtgcag tgaatgtgga
     1021 aaattctata gccacaagtc caaccttatc aaacattggc gtgttcatac tggagaaagg
     1081 ccttacaagt gcagcgactg tgggaaattt tttacccaat gctcaagcct catgcaacat
     1141 caaaaagttc acactggaga aaagcctttt aagtgcaatg aatgtgggag attctttaga
     1201 gagaattcca ccctagttag acatcagagg gttcacactg gagcaaagcc ttatgagtgc
     1261 agggaatgtg ggaaattttt tagccaaagc tcaaccctca tgcaacatcg aaaagttcac
     1321 attggagaaa agccttttaa gtgcaatgaa tgtgggagat tgtttagaga gaattccagc
     1381 cttgttaaac atcagagggt tcacactgga gcaaagcctt atgagtgcag ggaatgtggg
     1441 aagttttttc gccacaactc cagtcttttt aaacatcgaa ggattcacac tggagaaatg
     1501 cagtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1561 tgtggaaagg acgagaatag aatacctaag gcaatgca
//
